Literature DB >> 26063445

Sequence Analysis of lip R: A Good Method for Molecular Epidemiology of Clinical Isolates of Mycobacterium tuberculosis.

Samaneh Saedi1, Masoud Youssefi, Hadi Safdari, Saman Soleimanpour, Parviz Marouzi, Kiarash Ghazvini.   

Abstract

Advances in DNA sequencing have greatly enhanced the molecular epidemiology studies. In order to assess evolutionary and phylogenetic relation of Mycobacterium tuberculosis isolates several gene targets were evaluated. In this study, appropriate fragments of 5 highly variable genes (rpsL, mprA, lipR, katG, and fgd1 genes) were sequenced. The sequence data were analyzed with neighbor-joining method using mega and Geneious software. The phylogenetic trees analyzes revealed that the discriminatory power of lipR is much stronger than that observed in the other genes. lipR could distinguish between more clinical isolates. Therefore, lipR is a promising target for sequence analyzes of M. tuberculosis.

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Year:  2015        PMID： 26063445     DOI： 10.1007/s00284-015-0856-0

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  21 in total

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Authors:  Stephen D Lawn; Alimuddin I Zumla
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6.  Multiple-Locus Variable-Number Tandem-Repeats Analysis of Escherichia coli O157 using PCR multiplexing and multi-colored capillary electrophoresis.

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Authors:  Richard Truman; Amanda B Fontes; Antonio B De Miranda; Philip Suffys; Thomas Gillis
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

8.  A NEW AND RAPID METHOD FOR THE ISOLATION AND CULTIVATION OF TUBERCLE BACILLI DIRECTLY FROM THE SPUTUM AND FECES.

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Journal:  J Exp Med       Date:  1915-01-01       Impact factor: 14.307

9.  Extensive Genetic Diversity among Clinical Isolates of Mycobacterium tuberculosis in Central Province of Iran.

Authors:  Saman Soleimanpour; Daryoush Hamedi Asl; Keyvan Tadayon; Ali Asghar Farazi; Rouhollah Keshavarz; Kioomars Soleymani; Fereshteh Sadat Seddighinia; Nader Mosavari
Journal:  Tuberc Res Treat       Date:  2014-11-19

10.  Insight into microevolution of Yersinia pestis by clustered regularly interspaced short palindromic repeats.

Authors:  Yujun Cui; Yanjun Li; Olivier Gorgé; Mikhail E Platonov; Yanfeng Yan; Zhaobiao Guo; Christine Pourcel; Svetlana V Dentovskaya; Sergey V Balakhonov; Xiaoyi Wang; Yajun Song; Andrey P Anisimov; Gilles Vergnaud; Ruifu Yang
Journal:  PLoS One       Date:  2008-07-09       Impact factor: 3.240
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