Literature DB >> 26061244

Polarized Fluorescence Microscopy to Study Cytoskeleton Assembly and Organization in Live Cells.

Molly McQuilken1, Shalin B Mehta2, Amitabh Verma2, Grant Harris2, Rudolf Oldenbourg2, Amy S Gladfelter1.   

Abstract

The measurement of not only the location but also the organization of molecules in live cells is crucial to understanding diverse biological processes. Polarized light microscopy provides a nondestructive means to evaluate order within subcellular domains. When combined with fluorescence microscopy and GFP-tagged proteins, the approach can reveal organization within specific populations of molecules. This unit describes a protocol for measuring the architectural dynamics of cytoskeletal components using polarized fluorescence microscopy and OpenPolScope open-access software (http://www.openpolscope.org). The protocol describes installation of linear polarizers or a liquid crystal (LC) universal compensator, calibration of the system, polarized fluorescence imaging, and analysis. The use of OpenPolScope software and hardware allows for reliable, user-friendly image acquisition to measure and analyze polarized fluorescence.
Copyright © 2015 John Wiley & Sons, Inc.

Entities:  

Keywords:  OpenPolScope; cytoskeleton; image analysis; polarized fluorescence

Mesh：

Substances：

Year:  2015        PMID： 26061244      PMCID： PMC4529119          DOI： 10.1002/0471143030.cb0429s67

Source DB:  PubMed          Journal:  Curr Protoc Cell Biol        ISSN： 1934-2616


  14 in total

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Authors:  Shalin B Mehta; Michael Shribak; Rudolf Oldenbourg
Journal:  J Opt       Date:  2013-09-01       Impact factor: 2.516

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Authors:  Arthur Edelstein; Nenad Amodaj; Karl Hoover; Ron Vale; Nico Stuurman
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Authors:  Bradley S DeMay; Xiaobo Bai; Louisa Howard; Patricia Occhipinti; Rebecca A Meseroll; Elias T Spiliotis; Rudolf Oldenbourg; Amy S Gladfelter
Journal:  J Cell Biol       Date:  2011-06-13       Impact factor: 10.539

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Journal:  J Exp Biol       Date:  1950-09       Impact factor: 3.312
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  8 in total

Review 1.  Living Cells and Dynamic Molecules Observed with the Polarized Light Microscope: the Legacy of Shinya Inoué.

Authors:  Tomomi Tani; Michael Shribak; Rudolf Oldenbourg
Journal:  Biol Bull       Date:  2016-08       Impact factor: 1.818

2.  Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells.

Authors:  Shalin B Mehta; Molly McQuilken; Patrick J La Riviere; Patricia Occhipinti; Amitabh Verma; Rudolf Oldenbourg; Amy S Gladfelter; Tomomi Tani
Journal:  Proc Natl Acad Sci U S A       Date:  2016-09-27       Impact factor: 11.205

3.  Desmoglein 3 Order and Dynamics in Desmosomes Determined by Fluorescence Polarization Microscopy.

Authors:  Emily I Bartle; Tara M Urner; Siddharth S Raju; Alexa L Mattheyses
Journal:  Biophys J       Date:  2017-12-05       Impact factor: 4.033

Review 4.  Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates.

Authors:  Simon Alberti; Amy Gladfelter; Tanja Mittag
Journal:  Cell       Date:  2019-01-24       Impact factor: 41.582

5.  Cytokinesis in vertebrate cells initiates by contraction of an equatorial actomyosin network composed of randomly oriented filaments.

Authors:  Felix Spira; Sara Cuylen-Haering; Shalin Mehta; Matthias Samwer; Anne Reversat; Amitabh Verma; Rudolf Oldenbourg; Michael Sixt; Daniel W Gerlich
Journal:  Elife       Date:  2017-11-06       Impact factor: 8.140

6.  The ImageJ ecosystem: Open-source software for image visualization, processing, and analysis.

Authors:  Alexandra B Schroeder; Ellen T A Dobson; Curtis T Rueden; Pavel Tomancak; Florian Jug; Kevin W Eliceiri
Journal:  Protein Sci       Date:  2020-11-20       Impact factor: 6.725

7.  Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools.

Authors:  Alexey Bondar; Olga Rybakova; Josef Melcr; Jan Dohnálek; Petro Khoroshyy; Ondřej Ticháček; Štěpán Timr; Paul Miclea; Alina Sakhi; Vendula Marková; Josef Lazar
Journal:  Commun Biol       Date:  2021-02-12

8.  Analysis of Septin Reorganization at Cytokinesis Using Polarized Fluorescence Microscopy.

Authors:  Molly McQuilken; Maximilian S Jentzsch; Amitabh Verma; Shalin B Mehta; Rudolf Oldenbourg; Amy S Gladfelter
Journal:  Front Cell Dev Biol       Date:  2017-05-03
  8 in total

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