Literature DB >> 26057795

Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum.

Anastassios C Papageorgiou1, Duochuan Li2.   

Abstract

A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The crystal was found to belong to space group P2(1), with unit-cell parameters a = 64.1, b = 85.8, c = 68.5 Å, β = 93.1° and one molecule in the asymmetric unit.

Entities:  

Keywords:  cellulose; glucanase; in situ diffraction; seeding; thermophilic fungus

Mesh：

Substances：

Year:  2015        PMID： 26057795      PMCID： PMC4461330          DOI： 10.1107/S2053230X15006366

Source DB:  PubMed          Journal:  Acta Crystallogr F Struct Biol Commun        ISSN： 2053-230X            Impact factor:   1.056


  13 in total

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9.  Cloning, sequence and structure of a gene encoding an antifungal glucan 1,3-beta-glucosidase from Trichoderma atroviride (T. harzianum).

Authors:  B G Donzelli; M Lorito; F Scala; G E Harman
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