Marta Elena Álvarez-Argüelles1, María de Oña-Navarro2, Susana Rojo-Alba3, Magdalena Torrens-Muns4, Maria Luisa Junquera-Llaneza5, Jose Antonio-Boga6, Sonia Pérez-Castro7, Santiago Melón-García8. 1. Department of Microbiology, Hospital Universitario Central de Asturias, Oviedo, Spain. Electronic address: martaealvarez@gmail.com. 2. Department of Microbiology, Hospital Universitario Central de Asturias, Oviedo, Spain. Electronic address: maria.ona@sespa.princast.es. 3. Department of Microbiology, Hospital Universitario Central de Asturias, Oviedo, Spain. Electronic address: ssnrj4@gmail.com. 4. Department of Gynecology, Hospital Universitario Central de Asturias, Oviedo, Spain. Electronic address: magdalena.torrens@sespa.princast.es. 5. Sexually Transmitted Infections Unit, Hospital Monte Naranco, Oviedo, Spain. Electronic address: marisajunquera@gmail.com. 6. Department of Microbiology, Hospital Universitario Central de Asturias, Oviedo, Spain. Electronic address: jose.boga@sespa.princast.es. 7. Department of Microbiology, Complejo Hospitalario Universitario de Vigo, Spain. Electronic address: sonia.perez.castro@gmail.com. 8. Department of Microbiology, Hospital Universitario Central de Asturias, Oviedo, Spain. Electronic address: santiago.melon@sespa.princast.es.
Abstract
BACKGROUND AND PURPOSE: Surrogate markers such as viral load are necessary to follow the evolution of disease resulting from infection with Human Papilloma virus (HPV), especially in this era of vaccination. As such, this paper uses the automated system Cobas-4800-HPV to define viral load as number of HPV copies/cell and apply the results to clinical samples. STUDY DESIGN: A curve to determine viral load per cell was constructed from HPV plasmid and cell concentrations using the Cobas-4800-HPV system. According to these curves, HPV viral load was determined in 309 positive endocervical swabs (58 from patients with previous HPV-infection, 118 with current lesions and 133 symptom-free patients presenting for screening) from women attending gynaecology consultations from January to June 2013. RESULTS: In curves with r(2)≥0.95 the Cobas-4800-HPV system has a detection limit of 150 (2.18 log) viral copies, and the limit for β-globin corresponds to that of a single cell. In women reporting for screening, viral load was under 10(4) (4 log) copies/10(3) cells. For women with lesions or previous HPV infection loads were significantly higher particularly in the 30-45 year group (p=0.038). Elevated viral loads were especially noticeable in non-HPV 16/HPV 18. CONCLUSIONS: Automated system Cobas-4800-HPV is suitable for define viral load of HPV. Correlation between viral load and number of cells established. Higher viral load in women with disease, and those between 30 and 45 years. Increased viral load of non-16/18 high-risk HPV genotypes detected in patients with lesions compared to screening patients. A difference not observed for HPV 16/18, or in coinfections.
BACKGROUND AND PURPOSE: Surrogate markers such as viral load are necessary to follow the evolution of disease resulting from infection with Human Papilloma virus (HPV), especially in this era of vaccination. As such, this paper uses the automated system Cobas-4800-HPV to define viral load as number of HPV copies/cell and apply the results to clinical samples. STUDY DESIGN: A curve to determine viral load per cell was constructed from HPV plasmid and cell concentrations using the Cobas-4800-HPV system. According to these curves, HPV viral load was determined in 309 positive endocervical swabs (58 from patients with previous HPV-infection, 118 with current lesions and 133 symptom-free patients presenting for screening) from women attending gynaecology consultations from January to June 2013. RESULTS: In curves with r(2)≥0.95 the Cobas-4800-HPV system has a detection limit of 150 (2.18 log) viral copies, and the limit for β-globin corresponds to that of a single cell. In women reporting for screening, viral load was under 10(4) (4 log) copies/10(3) cells. For women with lesions or previous HPV infection loads were significantly higher particularly in the 30-45 year group (p=0.038). Elevated viral loads were especially noticeable in non-HPV 16/HPV 18. CONCLUSIONS: Automated system Cobas-4800-HPV is suitable for define viral load of HPV. Correlation between viral load and number of cells established. Higher viral load in women with disease, and those between 30 and 45 years. Increased viral load of non-16/18 high-risk HPV genotypes detected in patients with lesions compared to screening patients. A difference not observed for HPV 16/18, or in coinfections.
Authors: Lance R Peterson; Stephen A Young; Thomas E Davis; Zi-Xuam Wang; John Duncan; Christopher Noutsios; Oliver Liesenfeld; John C Osiecki; Michael A Lewinski Journal: J Clin Microbiol Date: 2017-09-27 Impact factor: 5.948
Authors: Ana Morilla; Santiago Melón; Marta E Álvarez-Argüelles; Edisa Armesto; Henar Villar; María de Oña Journal: PLoS One Date: 2017-06-02 Impact factor: 3.240
Authors: Constanza Gómez de Oña; Marta E Alvarez-Argüelles; Susana Rojo-Alba; Helena Casares; Mireia Arroyo; Julián Rodríguez; María de Oña; Santiago Melón Journal: Transl Pediatr Date: 2021-05