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Literature DB >> 26051

Minichromosome from BK virus as a template for transcription in vitro.

G Meneguzzi, P F Pignatti, G Barbanti-Brodano, G Milanesi.

Abstract

BK virus DNA can be extracted from virions as a nucleoprotein complex containing about 20 nucleosomes. Transcription of this "minichromosome" with Escherichia coli RNA polymerase indicates that both initiation and elongation of RNA chains are reduced by the presence of nucleosomes. Hybridization analysis of RNA made on the complex shows preferential transcription of one region of BK virus genome. No increase in strand selection is observed with respect to transcription of purified superhelical BK virus DNA.

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Year: 1978 PMID： 26051 PMCID： PMC411421 DOI： 10.1073/pnas.75.3.1126

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

1. Animal DNA-dependent RNA polymerases. Analysis of the RNAs synthesized on Simian virus 40 superhelical DNA by mammalian RNA polymerases AI and B.

Authors: J L Mandel; P Chambon
Journal: Eur J Biochem Date: 1974-01-16

Minichromosome from BK virus as a template for transcription in vitro.

1. Animal DNA-dependent RNA polymerases. Analysis of the RNAs synthesized on Simian virus 40 superhelical DNA by mammalian RNA polymerases AI and B.

2. Polyoma viral DNA replicated as a nucleoprotein complex in close association with the host cell chromatin.

3. Properties of the polyoma virus transcription complex obtained from mouse nuclei.

4. Prevalence in Italy of antibodies to a new human papovavirus (BK virus).

5. A new preparation method for dark-field electron microscopy of biomacromolecules.

6. Template activities of the phi X-174 replicative allomorphic deoxyribonucleic acids.

7. The ribonuclease activity of crystallized pancreatic deoxyribonuclease.

8. Template specificity of transcription during sporulation of Bacillus subtilis.

9. Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis.

10. Malignant transformation of BHK21 clone 13 cells by BK virus--a human papovavirus.

1. Transcription of nucleosomal DNA in SV40 minichromosomes by eukaryotic and prokaryotic RNA polymerases.

2. Inoculation with BK virus may break immunological tolerance to histone and DNA antigens.

3. Analysis of herpes simplex virus nucleoprotein complexes extracted from infected cells.

4. The nature of the interaction of nucleosomes with a eukaryotic RNA polymerase II.

5. Inhibitory effect of gamma interferon on BK virus gene expression and replication.

Review 6. The role of polyomaviruses in human disease.

7. Global effects of BKV infection on gene expression in human primary kidney epithelial cells.

8. Efficient transcription of a compact nucleoprotein complex isolated from purified simian virus 40 virions.

9. Integrated and free viral DNA in hamster tumors induced by BK virus.

Review 10. JC virus: an oncogenic virus in animals and humans?