Literature DB >> 2604724

Conformational analysis of PKI(5-22)amide, the active inhibitory fragment of the inhibitor protein of the cyclic AMP-dependent protein kinase.

J Reed1, J S De Ropp, J Trewhella, D B Glass, W K Liddle, E M Bradbury, V Kinzel, D A Walsh.   

Abstract

Fourier-transform i.r. spectroscopy, 1H-n.m.r. spectroscopy and X-ray scattering were used to study the conformation and shape of the peptide PKI(5-22)amide, which contains the active site of the inhibitor protein of the cyclic AMP-dependent protein kinase [Cheng, Van Pattern, Smith & Walsh (1985) Biochem. J. 231, 655-661]. The X-ray-scattering solution studies show that the peptide has a compact structure with Rg 0.9 nm (9.0 A) and a linear maximum dimension of 2.5 nm (25A). Compatible with this, Fourier-transform i.r. and n.m.r. determinations indicate that the peptide contains approx. 26% alpha-helix located in the N-terminal one-third of the molecule. This region contains the phenylalanine residue that is one essential recognition determinant for high-affinity binding to the protein kinase catalytic site.

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Year:  1989        PMID： 2604724      PMCID： PMC1133591          DOI： 10.1042/bj2640371

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  22 in total

1.  Isolation and properties of the rabbit skeletal muscle protein inhibitor of adenosine 3',5'-monophosphate dependent protein kinases.

Authors:  J G Demaille; K A Peters; E H Fischer
Journal:  Biochemistry       Date:  1977-07-12       Impact factor: 3.162

2.  Prediction of beta-turns.

Authors:  P Y Chou; G D Fasman
Journal:  Biophys J       Date:  1979-06       Impact factor: 4.033

Review 3.  Empirical predictions of protein conformation.

Authors:  P Y Chou; G D Fasman
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

4.  Krebs EG: Purification and characterization of a protein inhibitor of adenosine 3',5'-monophosphate-dependent protein kinases.

Authors:  D A Walsh; C D Ashby; C Gonzalez; D Calkins; E H Fischer
Journal:  J Biol Chem       Date:  1971-04-10       Impact factor: 5.157

5.  Structural interpretation of vicinal proton-proton coupling constants 3JH alpha H beta in the basic pancreatic trypsin inhibitor measured by two-dimensional J-resolved NMR spectroscopy.

Authors:  K Nagayama; K Wüthrich
Journal:  Eur J Biochem       Date:  1981-04

6.  Dimerization by colicin E3 in the absence of immunity protein.

Authors:  B L Levinson; C A Pickover; F M Richards
Journal:  J Biol Chem       Date:  1983-09-25       Impact factor: 5.157

7.  Proton nuclear magnetic resonance investigation of synthetic calcium-binding peptides.

Authors:  J Gariépy; B D Sykes; R E Reid; R S Hodges
Journal:  Biochemistry       Date:  1982-03-30       Impact factor: 3.162

8.  Nuclear magnetic resonance studies of the conformation and kinetics of the peptide-substrate at the active site of bovine heart protein kinase.

Authors:  J Granot; A S Mildvan; H N Bramson; N Thomas; E T Kaiser
Journal:  Biochemistry       Date:  1981-02-03       Impact factor: 3.162

9.  Protein kinase inhibitor-(6-22)-amide peptide analogs with standard and nonstandard amino acid substitutions for phenylalanine 10. Inhibition of cAMP-dependent protein kinase.

Authors:  D B Glass; L J Lundquist; B M Katz; D A Walsh
Journal:  J Biol Chem       Date:  1989-08-25       Impact factor: 5.157

10.  Purification and characterization of 3':5'-cyclic GMP-dependent protein kinase.

Authors:  G N Gill; K E Holdy; G M Walton; C B Kanstein
Journal:  Proc Natl Acad Sci U S A       Date:  1976-11       Impact factor: 11.205
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  7 in total

1.  Molecular cloning of a rat testis form of the inhibitor protein of cAMP-dependent protein kinase.

Authors:  S M Van Patten; D C Ng; J P Th'ng; K L Angelos; A J Smith; D A Walsh
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-15       Impact factor: 11.205

2.  Selective disruption of the AKAP signaling complexes.

Authors:  Eileen J Kennedy; John D Scott
Journal:  Methods Mol Biol       Date:  2015

3.  Two well-defined motifs in the cAMP-dependent protein kinase inhibitor (PKIalpha) correlate with inhibitory and nuclear export function.

Authors:  J A Hauer; P Barthe; S S Taylor; J Parello; A Padilla
Journal:  Protein Sci       Date:  1999-03       Impact factor: 6.725

4.  A dual-specificity isoform of the protein kinase inhibitor PKI produced by alternate gene splicing.

Authors:  Priyadarsini Kumar; Donal A Walsh
Journal:  Biochem J       Date:  2002-03-15       Impact factor: 3.857

5.  Biochemical Analysis of AKAP-Anchored PKA Signaling Complexes.

Authors:  Dominic P Byrne; Mitchell H Omar; Eileen J Kennedy; Patrick A Eyers; John D Scott
Journal:  Methods Mol Biol       Date:  2022

6.  Conformationally constrained analogs of protein kinase inhibitor (6-22)amide: effect of turn structures in the center of the peptide on inhibition of cAMP-dependent protein kinase.

Authors:  D B Glass; J Trewhella; R D Mitchell; D A Walsh
Journal:  Protein Sci       Date:  1995-03       Impact factor: 6.725

7.  Phosphotransferase and substrate binding mechanism of the cAMP-dependent protein kinase catalytic subunit from porcine heart as deduced from the 2.0 A structure of the complex with Mn2+ adenylyl imidodiphosphate and inhibitor peptide PKI(5-24).

Authors:  D Bossemeyer; R A Engh; V Kinzel; H Ponstingl; R Huber
Journal:  EMBO J       Date:  1993-03       Impact factor: 11.598
  7 in total

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