Evaluation of phytoconstituents of three plants Acorus calamus linn. Artemisia absinthium Linn and Bergenia himalaica boriss by FTIR spectroscopic analysis.

Qualitative and quantitative analysis of plant extracts can be achieved by using different spectroscopic techniques. In current research work we deal with the nature of the absorption and spectra of extract of Acorus calamus, Artemisia absinthium and Bergenia himalaica using FTIR spectroscopic technique. The present study was focused on standardization of crude extracts by utilization of infrared light. The spectra of crude extracts (A. calamus, A. absinthium and B. himalaica) displayed very clear diagnostic peaks of functional groups i.e. O-H alcoholic/acid, C-H alkyl & aromatic ring, carbonyl, and C-O-C groups. The spectra of all the three plants did not show any peak at 2220-2260 cm(-1), which is indicative of the absence of nitrogen containing groups. These results exhibited that these plants does not contain any toxic substances.