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Literature DB >> 26039892

Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture.

Stefanie Utech¹, Radivoje Prodanovic^1,2, Angelo S Mao¹, Raluca Ostafe^1,3, David J Mooney^1,4, David A Weitz^1,5.

Abstract

Monodisperse alginate microgels (10-50 μm) are created via droplet-based microfluidics by a novel crosslinking procedure. Ionic crosslinking of alginate is induced by release of chelated calcium ions. The process separates droplet formation and gelation reaction enabling excellent control over size and homogeneity under mild reaction conditions. Living mesenchymal stem cells are encapsulated and cultured in the generated 3D microenvironments.

Entities: Chemical Gene

Keywords: alginate; cells; encapsulation; microfluidics; microgels

Mesh：

Substances：

Year: 2015 PMID： 26039892 PMCID： PMC4529809 DOI： 10.1002/adhm.201500021

Source DB: PubMed Journal: Adv Healthc Mater ISSN： 2192-2640 Impact factor: 9.933

27 in total

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Authors: Gorka Orive; Rosa María Hernández; Alicia R Gascón; Riccardo Calafiore; Thomas M S Chang; Paul De Vos; Gonzalo Hortelano; David Hunkeler; Igor Lacík; A M James Shapiro; José Luis Pedraz
Journal: Nat Med Date: 2003-01 Impact factor: 53.440

Review 2. Microfluidic encapsulation of cells in polymer microgels.

Authors: Diego Velasco; Ethan Tumarkin; Eugenia Kumacheva
Journal: Small Date: 2012-03-29 Impact factor: 13.281

3. Microfluidic production of biopolymer microcapsules with controlled morphology.

Authors: Hong Zhang; Ethan Tumarkin; Raheem Peerani; Zhihong Nie; Ruby May A Sullan; Gilbert C Walker; Eugenia Kumacheva
Journal: J Am Chem Soc Date: 2006-09-20 Impact factor: 15.419

4. A microfluidic approach to encapsulate living cells in uniform alginate hydrogel microparticles.

Authors: Carlos J Martinez; Jin Woong Kim; Congwang Ye; Idelise Ortiz; Amy C Rowat; Manuel Marquez; David Weitz
Journal: Macromol Biosci Date: 2012-02-07 Impact factor: 4.979

Review 5. The use of micro- and nanospheres as functional components for bone tissue regeneration.

Authors: Huanan Wang; Sander C G Leeuwenburgh; Yubao Li; John A Jansen
Journal: Tissue Eng Part B Rev Date: 2011-09-23 Impact factor: 6.389

6. Shape controllable microgel particles prepared by microfluidic combining external ionic crosslinking.

Authors: Yuandu Hu; Qin Wang; Jianying Wang; Jintao Zhu; Hong Wang; Yajiang Yang
Journal: Biomicrofluidics Date: 2012-05-18 Impact factor: 2.800

7. Biocompatible surfactants for water-in-fluorocarbon emulsions.

Authors: C Holtze; A C Rowat; J J Agresti; J B Hutchison; F E Angilè; C H J Schmitz; S Köster; H Duan; K J Humphry; R A Scanga; J S Johnson; D Pisignano; D A Weitz
Journal: Lab Chip Date: 2008-09-02 Impact factor: 6.799

8. Harnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate.

Authors: Nathaniel Huebsch; Praveen R Arany; Angelo S Mao; Dmitry Shvartsman; Omar A Ali; Sidi A Bencherif; José Rivera-Feliciano; David J Mooney
Journal: Nat Mater Date: 2010-04-25 Impact factor: 43.841

9. Droplet-based microfluidic platforms for the encapsulation and screening of Mammalian cells and multicellular organisms.

Authors: Jenifer Clausell-Tormos; Diana Lieber; Jean-Christophe Baret; Abdeslam El-Harrak; Oliver J Miller; Lucas Frenz; Joshua Blouwolff; Katherine J Humphry; Sarah Köster; Honey Duan; Christian Holtze; David A Weitz; Andrew D Griffiths; Christoph A Merten
Journal: Chem Biol Date: 2008-05

10. Alginate hydrogels as synthetic extracellular matrix materials.

Authors: J A Rowley; G Madlambayan; D J Mooney
Journal: Biomaterials Date: 1999-01 Impact factor: 12.479

47 in total

Review 1. Applications of alginate microspheres in therapeutics delivery and cell culture: Past, present and future.

Authors: Dinesh Dhamecha; Rachel Movsas; Ugene Sano; Jyothi U Menon
Journal: Int J Pharm Date: 2019-08-14 Impact factor: 5.875

2. Single cell-laden protease-sensitive microniches for long-term culture in 3D.

Authors: Philipp S Lienemann; Torsten Rossow; Angelo S Mao; Queralt Vallmajo-Martin; Martin Ehrbar; David J Mooney
Journal: Lab Chip Date: 2017-02-14 Impact factor: 6.799

Review 3. Methods for Generating Hydrogel Particles for Protein Delivery.

Authors: Allen L Liu; Andrés J García
Journal: Ann Biomed Eng Date: 2016-05-09 Impact factor: 3.934

4. A Fluidic Culture Platform for Spatially Patterned Cell Growth, Differentiation, and Cocultures.

Authors: Josephine Lembong; Max J Lerman; Tami J Kingsbury; Curt I Civin; John P Fisher
Journal: Tissue Eng Part A Date: 2018-07-13 Impact factor: 3.845

Review 5. Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics.

Authors: Sébastien Sart; Spiros N Agathos
Journal: Mol Biotechnol Date: 2018-11 Impact factor: 2.695

Review 6. Generation and manipulation of hydrogel microcapsules by droplet-based microfluidics for mammalian cell culture.

Authors: Haishui Huang; Yin Yu; Yong Hu; Xiaoming He; O Berk Usta; Martin L Yarmush
Journal: Lab Chip Date: 2017-05-31 Impact factor: 6.799

Review 10. Cell armor for protection against environmental stress: Advances, challenges and applications in micro- and nanoencapsulation of mammalian cells.

Authors: Onur Hasturk; David L Kaplan
Journal: Acta Biomater Date: 2018-11-24 Impact factor: 8.947