Ahmet Sahbaz1, Oner Aynioglu2, Hatice Isik3, Banu Dogan Gun4, Osman Cengil5, Onur Erol6. 1. School of Medicine, Department of Obstetrics and Gynecology, Zonguldak Bulent Ecevit University, Kozlu, Zonguldak, Turkey. drsahbazahmet@yahoo.com. 2. School of Medicine, Department of Obstetrics and Gynecology, Zonguldak Bulent Ecevit University, Kozlu, Zonguldak, Turkey. o.aynoglu@yahoo.com. 3. School of Medicine, Department of Obstetrics and Gynecology, Zonguldak Bulent Ecevit University, Kozlu, Zonguldak, Turkey. k.hgonbe@gmail.com. 4. School of Medicine, Department of Pathology, Zonguldak Bulent Ecevit University, Kozlu, Zonguldak, Turkey. banudogangun@yahoo.com. 5. Experimental Animal Research Laboratory, School of Medicine, Zonguldak Bulent Ecevit University, Kozlu, Zonguldak, Turkey. osmancengil@gmail.com. 6. Department of Gynecological Oncology, Antalya Education and Research Hospital, Antalya, Turkey. dronurerol@hotmail.com.
Abstract
PURPOSE: This study tested the ability of pycnogenol, an extract from the bark of the French maritime pine (Pinus pinaster), to prevent intra-abdominal adhesions. METHODS: Thirty female Wistar albino rats were separated randomly into three equal groups: Group (1) the control group, which underwent surgery, but was given no drug; Group (2) given 10 mg/kg of pycnogenol dissolved in normal saline intraperitoneally for 10 days after surgery; and Group (3) given 0.1 mL of normal saline for 10 days intraperitoneally after surgery. On post-operative day 10, all of the animals were killed and any adhesions were evaluated macroscopically and histopathologically. RESULTS: The macroscopic adhesion scores (mean ± SD) for Groups 1, 2, and 3 were 2.5 ± 0.53, 0.60 ± 0.70, and 2.0 ± 0.82, respectively. The macroscopic adhesion score was significantly lower in Group 2 than in Groups 1 and 3 (p < 0.001). All three components of the histopathological evaluation (inflammation, fibrosis, and neovascularization) were significantly lower in Group 2 than in Groups 1 or 3 (p < 0.001, p < 0.001, and p = 0.004, respectively). CONCLUSIONS: Pycnogenol was found to be effective at preventing surgery-related adhesions in an animal model.
PURPOSE: This study tested the ability of pycnogenol, an extract from the bark of the French maritime pine (Pinus pinaster), to prevent intra-abdominal adhesions. METHODS: Thirty female Wistar albino rats were separated randomly into three equal groups: Group (1) the control group, which underwent surgery, but was given no drug; Group (2) given 10 mg/kg of pycnogenol dissolved in normal saline intraperitoneally for 10 days after surgery; and Group (3) given 0.1 mL of normal saline for 10 days intraperitoneally after surgery. On post-operative day 10, all of the animals were killed and any adhesions were evaluated macroscopically and histopathologically. RESULTS: The macroscopic adhesion scores (mean ± SD) for Groups 1, 2, and 3 were 2.5 ± 0.53, 0.60 ± 0.70, and 2.0 ± 0.82, respectively. The macroscopic adhesion score was significantly lower in Group 2 than in Groups 1 and 3 (p < 0.001). All three components of the histopathological evaluation (inflammation, fibrosis, and neovascularization) were significantly lower in Group 2 than in Groups 1 or 3 (p < 0.001, p < 0.001, and p = 0.004, respectively). CONCLUSIONS:Pycnogenol was found to be effective at preventing surgery-related adhesions in an animal model.
Entities:
Keywords:
Adhesions; Female; Intra-abdominal; Pycnogenol; Rat