| Literature DB >> 26025458 |
Licheng Liu1, Yang Sun2, Brima Kargbo3, Chuntao Zhang4, Huahua Feng5, Huijun Lu2, Wenseng Liu2, Chengyu Wang2, Yi Hu2, Yongqiang Deng2, Jiafu Jiang2, Xiaoping Kang1, Honglei Yang5, Yongqiang Jiang1, Yinhui Yang1, David Kargbo6, Jun Qian7, Weijun Chen8.
Abstract
During the 2014 Ebola virus disease (EVD) outbreak, a real-time quantitative polymerase chain reaction was established to detect and identify the Zaire Ebola virus. We describe the use of this assay to screen 315 clinical samples from EVD suspected person in Sierra Leone. The detection rate in blood samples was 77.81% (207/266), and there were relatively higher detection rate (79.32% and 81.42%, respectively) during the first two weeks after onset of symptoms. In the two weeks that followed, the detection rate declined to 66.67% and 25.00%, respectively. There was the highest virus load at the first week and then decreased. The detection rate in swab samples was 89.79% (44/49). This may be benefit from the included patients. 46 of 49 swab samples were collected from died patients. Taken together, the results presented here indicate that the assay specifically and sensitively detects Zaire Ebola virus.Entities:
Keywords: Ebola virus disease; Real-time reverse transcription polymerase chain reaction (rRT-PCR); Zaire Ebola virus
Mesh：
Year: 2015 PMID： 26025458 DOI： 10.1016/j.jviromet.2015.05.005
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014