OBJECTIVE: Oxidative stress events have been implicated to occur and facilitate multiple failure modes of intracortical microelectrodes. The goal of the present study was to evaluate the ability of a sustained concentration of an anti-oxidant and to reduce oxidative stress-mediated neurodegeneration for the application of intracortical microelectrodes. APPROACH: Non-functional microelectrodes were implanted into the cortex of male Sprague Dawley rats for up to sixteen weeks. Half of the animals received a daily intraperitoneal injection of the natural anti-oxidant resveratrol, at 30 mg kg(-1). The study was designed to investigate the biodistribution of the resveratrol, and the effects on neuroinflammation/neuroprotection following device implantation. MAIN RESULTS: Daily maintenance of a sustained range of resveratrol throughout the implantation period resulted in fewer degenerating neurons in comparison to control animals at both two and sixteen weeks post implantation. Initial and chronic improvements in neuronal viability in resveratrol-dosed animals were correlated with significant reductions in local superoxide anion accumulation around the implanted device at two weeks after implantation. Controls, receiving only saline injections, were also found to have reduced amounts of accumulated superoxide anion locally and less neurodegeneration than controls at sixteen weeks post-implantation. Despite observed benefits, thread-like adhesions were found between the liver and diaphragm in resveratrol-dosed animals. SIGNIFICANCE: Overall, our chronic daily anti-oxidant dosing scheme resulted in improvements in neuronal viability surrounding implanted microelectrodes, which could result in improved device performance. However, due to the discovery of thread-like adhesions, further work is still required to optimize a chronic anti-oxidant dosing regime for the application of intracortical microelectrodes.
OBJECTIVE: Oxidative stress events have been implicated to occur and facilitate multiple failure modes of intracortical microelectrodes. The goal of the present study was to evaluate the ability of a sustained concentration of an anti-oxidant and to reduce oxidative stress-mediated neurodegeneration for the application of intracortical microelectrodes. APPROACH: Non-functional microelectrodes were implanted into the cortex of male Sprague Dawley rats for up to sixteen weeks. Half of the animals received a daily intraperitoneal injection of the natural anti-oxidant resveratrol, at 30 mg kg(-1). The study was designed to investigate the biodistribution of the resveratrol, and the effects on neuroinflammation/neuroprotection following device implantation. MAIN RESULTS: Daily maintenance of a sustained range of resveratrol throughout the implantation period resulted in fewer degenerating neurons in comparison to control animals at both two and sixteen weeks post implantation. Initial and chronic improvements in neuronal viability in resveratrol-dosed animals were correlated with significant reductions in local superoxide anion accumulation around the implanted device at two weeks after implantation. Controls, receiving only saline injections, were also found to have reduced amounts of accumulated superoxide anion locally and less neurodegeneration than controls at sixteen weeks post-implantation. Despite observed benefits, thread-like adhesions were found between the liver and diaphragm in resveratrol-dosed animals. SIGNIFICANCE: Overall, our chronic daily anti-oxidant dosing scheme resulted in improvements in neuronal viability surrounding implanted microelectrodes, which could result in improved device performance. However, due to the discovery of thread-like adhesions, further work is still required to optimize a chronic anti-oxidant dosing regime for the application of intracortical microelectrodes.
Authors: Leigh R Hochberg; Daniel Bacher; Beata Jarosiewicz; Nicolas Y Masse; John D Simeral; Joern Vogel; Sami Haddadin; Jie Liu; Sydney S Cash; Patrick van der Smagt; John P Donoghue Journal: Nature Date: 2012-05-16 Impact factor: 49.962
Authors: Steven M Wellman; James R Eles; Kip A Ludwig; John P Seymour; Nicholas J Michelson; William E McFadden; Alberto L Vazquez; Takashi D Y Kozai Journal: Adv Funct Mater Date: 2017-07-19 Impact factor: 18.808
Authors: Andrew J Shoffstall; Jen E Paiz; David M Miller; Griffin M Rial; Mitchell T Willis; Dhariyat M Menendez; Stephen R Hostler; Jeffrey R Capadona Journal: J Neural Eng Date: 2017-12-05 Impact factor: 5.379
Authors: Monika Goss-Varley; Andrew J Shoffstall; Keith R Dona; Justin A McMahon; Sydney C Lindner; Evon S Ereifej; Jeffrey R Capadona Journal: J Vis Exp Date: 2018-08-18 Impact factor: 1.355
Authors: John K Hermann; Madhumitha Ravikumar; Andrew J Shoffstall; Evon S Ereifej; Kyle M Kovach; Jeremy Chang; Arielle Soffer; Chun Wong; Vishnupriya Srivastava; Patrick Smith; Grace Protasiewicz; Jingle Jiang; Stephen M Selkirk; Robert H Miller; Steven Sidik; Nicholas P Ziats; Dawn M Taylor; Jeffrey R Capadona Journal: J Neural Eng Date: 2018-04 Impact factor: 5.379
Authors: Jessica K Nguyen; Mehdi Jorfi; Kelly L Buchanan; Daniel J Park; E Johan Foster; Dustin J Tyler; Stuart J Rowan; Christoph Weder; Jeffrey R Capadona Journal: Acta Biomater Date: 2015-11-06 Impact factor: 8.947
Authors: Jessica D Falcone; Sheridan L Carroll; Tarun Saxena; Dev Mandavia; Alexus Clark; Varun Yarabarla; Ravi V Bellamkonda Journal: Front Bioeng Biotechnol Date: 2018-03-27