M E Łysakowska1, A Ciebiada-Adamiec2, M Sienkiewicz3, J Sokołowski4, K Banaszek4. 1. Department of Microbiology and Medical Laboratory Immunology, Medical University of Lodz, Lodz, Poland. 2. Medical Diagnostic Laboratory Center, Polish Mother's Memory Institute, Medical University of Lodz, Lodz, Poland. 3. Department of Environmental Biology, Medical University of Lodz, Lodz, Poland. 4. Department of General Dentistry, Medical University of Lodz, Lodz, Poland.
Abstract
AIM: To examine the presence of microbial species in primary and secondary infections and identify the signs and symptoms associated with them. METHODOLOGY: A total number of 37 root canals from 33 patients undergoing root canal treatment were selected. Samples were taken using a sterile paper point following chemomechanical canal instrumentation. Microbiological identification was performed by macromorphological, micromorphological and commercial biochemical tests (ATB system). The antibiotic susceptibility profile of isolated Enterococcus, Streptococcus and Propionibacterium spp. was evaluated. RESULTS: A total number of 54 species were isolated. The most common was E. faecalis followed by Propionibacterium acnes and Streptococcus spp. Five multidrug-resistant enterococcal strains were isolated. However, resistance to linezolid or glycopeptides was not found. Radiolucency was observed significantly more often in symptomatic infections (P = 0.0061) caused by Actinomyces spp. than in asymptomatic ones. CONCLUSIONS: Enterococci and streptococci were the most commonly isolated bacteria from root canals. The resistance of enterococci to many antimicrobials may pose a problem in their eradication.
AIM: To examine the presence of microbial species in primary and secondary infections and identify the signs and symptoms associated with them. METHODOLOGY: A total number of 37 root canals from 33 patients undergoing root canal treatment were selected. Samples were taken using a sterile paper point following chemomechanical canal instrumentation. Microbiological identification was performed by macromorphological, micromorphological and commercial biochemical tests (ATB system). The antibiotic susceptibility profile of isolated Enterococcus, Streptococcus and Propionibacterium spp. was evaluated. RESULTS: A total number of 54 species were isolated. The most common was E. faecalis followed by Propionibacterium acnes and Streptococcus spp. Five multidrug-resistant enterococcal strains were isolated. However, resistance to linezolid or glycopeptides was not found. Radiolucency was observed significantly more often in symptomatic infections (P = 0.0061) caused by Actinomyces spp. than in asymptomatic ones. CONCLUSIONS: Enterococci and streptococci were the most commonly isolated bacteria from root canals. The resistance of enterococci to many antimicrobials may pose a problem in their eradication.
Authors: Mario Dioguardi; Mario Alovisi; Vito Crincoli; Riccardo Aiuto; Giancarlo Malagnino; Cristian Quarta; Enrica Laneve; Diego Sovereto; Lucio Lo Russo; Giuseppe Troiano; Lorenzo Lo Muzio Journal: J Clin Med Date: 2020-03-09 Impact factor: 4.241
Authors: Fabian Cieplik; Andreas Pummer; Christoph Leibl; Johannes Regensburger; Gottfried Schmalz; Wolfgang Buchalla; Karl-Anton Hiller; Tim Maisch Journal: Front Microbiol Date: 2016-06-15 Impact factor: 5.640