Ehsan Soltaninejad1, Mohammad Hossein Nicknam2, Mohsen Nafar3, Pedram Ahmadpoor3, Fatemeh Pourrezagholi3, Mohammad Hossein Sharbafi4, Morteza Hosseinzadeh4, Farshad Foroughi5, Mir Saeed Yekaninejad6, Tayyeb Bahrami5, Ehsan Sharif-Paghaleh4, Aliakbar Amirzargar7. 1. Department of Immunology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran; Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran. 3. Chronic Kidney Disease Research Center and Department of Nephrology, Shahid Labbafinejad Medical Center and Shahid Beheshti University of Medical Sciences, Tehran, Iran. 4. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 5. Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran. 6. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 7. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: amirzara@sina.tums.ac.ir.
Abstract
BACKGROUND: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA). METHODS: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA. RESULTS: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients. CONCLUSION: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.
BACKGROUND: MicroRNAs (miRNAs) regulate most of encoding genes and protein. In this study, we aimed to investigate the expression levels of miR-142-5p, miR-142-3p, miR-155 and miR-223 in paired biopsy and peripheral blood mononuclear cell (PBMC) samples of renal allograft recipients with acute T-cell mediated rejection (ATCMR), compared with normal allografts (NA). METHODS: In this study, the expression levels of individual miRNAs were determined in biopsy and PBMC samples of 17 recipients with ATCMR and 18 recipients with NA. RESULTS: Our results showed that the intragraft expression levels of all studied miRNAs were significantly higher in ATCMR than NA. However, regarding the PBMC samples, miR-142-3p and miR-223 were significantly increased in ATCMR than NA. Receiver operating characteristic (ROC) analysis showed that miR-142-5p, miR-142-3p, miR-155 and miR-223 in biopsy samples and miR-142-3p and miR-223 in PBMC samples could discriminate ATCMR from NA recipients. CONCLUSION: It has been reported that high intragraft expressions of miRNAs have a profound role in the pathogenesis of ATCMR process. Our results showed that high expression of all the studied miRNAs in biopsies and miR-142-3p and miR-223 in PBMC samples could be used as suggestive diagnostic tools to discriminate ATCMR patients from NA.
Authors: Kristien J Ledeganck; Els M Gielis; Daniel Abramowicz; Peter Stenvinkel; Paul G Shiels; Amaryllis H Van Craenenbroeck Journal: Clin J Am Soc Nephrol Date: 2019-01-02 Impact factor: 8.237
Authors: Roel Bijkerk; Barend W Florijn; Meriem Khairoun; Jacques M G J Duijs; Gurbey Ocak; Aiko P J de Vries; Alexander F Schaapherder; Marko J K Mallat; Johan W de Fijter; Ton J Rabelink; Anton Jan van Zonneveld; Marlies E J Reinders Journal: Transplant Direct Date: 2017-06-19
Authors: Pauline Erpicum; Oriane Hanssen; Laurent Weekers; Pierre Lovinfosse; Paul Meunier; Luaba Tshibanda; Jean-Marie Krzesinski; Roland Hustinx; François Jouret Journal: Clin Kidney J Date: 2016-09-06
Authors: Els M Gielis; Jacqueline D H Anholts; Els van Beelen; Geert W Haasnoot; Hans W De Fijter; Ingeborg Bajema; Sebastiaan Heidt; Mathijs van de Vrie; Luuk B Hilbrands; Marko J K Mallat; Kristien J Ledeganck; Frans H J Claas; Michael Eikmans Journal: Transplant Direct Date: 2021-06-10