Sepideh Zununi Vahed1, Ahmad Poursadegh Zonouzi2, Hossein Ghanbarian3, Morteza Ghojazadeh4, Nasser Samadi5, Yadollah Omidi6, Mohammadreza Ardalan7. 1. Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran. 2. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran. 3. School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran. 4. Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran. 5. School of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran. 6. Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Islamic Republic of Iran. 7. Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Islamic Republic of Iran. ardalan34@yahoo.com.
Abstract
BACKGROUND: The discovery of circulating microRNAs (miRNAs), as potential noninvasive diagnostic biomarkers, has opened new avenues of research for identifying patients with chronic failure in renal transplantation. The present study aimed to investigate the expression levels of four immune-related miRNAs (miR-21, miR-31, miR-142-3p and miR-155) in plasma samples of renal recipients. METHODS: The plasma expression levels of the miRNAs were evaluated by quantitative real-time PCR (qPCR) in 53 renal recipients with long-term stable allograft function, SGF (N = 27), and with biopsy-proven interstitial fibrosis and tubular atrophy (IFTA) (N = 26) and also healthy controls (N = 15). The possible correlation between clinical parameters and the circulating miRNAs and the receiver-operating characteristic (ROC) analysis were performed. RESULTS: Our results showed that expression of miR-21 (p = 0.023), miR-142-3p (p = 0.048) and miR-155 (p = 0.005) was significantly upregulated in plasma samples of recipients with IFTA in comparison with SGF and healthy control groups. Concentration of miR-21 (∆Ct value) in plasma was negatively correlated with creatinine (r = -0.432, p = 0.028) and positively correlated with eGFR (r = 0.423, p = 0.031). The multivariate ROC curve analysis indicated that miR-21, miR-142-3p and miR-155 in plasma samples could discriminate almost most of the IFTA patients (area under curve = 0.802, sensitivity = 81%, specificity = 92%). CONCLUSION: Our data suggested that altered expression of miR-21, miR-142-3p and miR-155 in plasma samples may be associated with renal dysfunction and can be used for graft monitoring.
BACKGROUND: The discovery of circulating microRNAs (miRNAs), as potential noninvasive diagnostic biomarkers, has opened new avenues of research for identifying patients with chronic failure in renal transplantation. The present study aimed to investigate the expression levels of four immune-related miRNAs (miR-21, miR-31, miR-142-3p and miR-155) in plasma samples of renal recipients. METHODS: The plasma expression levels of the miRNAs were evaluated by quantitative real-time PCR (qPCR) in 53 renal recipients with long-term stable allograft function, SGF (N = 27), and with biopsy-proven interstitial fibrosis and tubular atrophy (IFTA) (N = 26) and also healthy controls (N = 15). The possible correlation between clinical parameters and the circulating miRNAs and the receiver-operating characteristic (ROC) analysis were performed. RESULTS: Our results showed that expression of miR-21 (p = 0.023), miR-142-3p (p = 0.048) and miR-155 (p = 0.005) was significantly upregulated in plasma samples of recipients with IFTA in comparison with SGF and healthy control groups. Concentration of miR-21 (∆Ct value) in plasma was negatively correlated with creatinine (r = -0.432, p = 0.028) and positively correlated with eGFR (r = 0.423, p = 0.031). The multivariate ROC curve analysis indicated that miR-21, miR-142-3p and miR-155 in plasma samples could discriminate almost most of the IFTApatients (area under curve = 0.802, sensitivity = 81%, specificity = 92%). CONCLUSION: Our data suggested that altered expression of miR-21, miR-142-3p and miR-155 in plasma samples may be associated with renal dysfunction and can be used for graft monitoring.
Authors: Richard Danger; Annaïck Pallier; Magali Giral; Marc Martínez-Llordella; Juan José Lozano; Nicolas Degauque; Alberto Sanchez-Fueyo; Jean-Paul Soulillou; Sophie Brouard Journal: J Am Soc Nephrol Date: 2012-01-26 Impact factor: 10.121
Authors: M J Scian; D G Maluf; K G David; K J Archer; J L Suh; A R Wolen; M U Mba; H D Massey; A L King; T Gehr; A Cotterell; M Posner; V Mas Journal: Am J Transplant Date: 2011-07-27 Impact factor: 8.086
Authors: Brandi L Carofino; Kayla M Dinshaw; Pui Yan Ho; Christophe Cataisson; Aleksandra M Michalowski; Andrew Ryscavage; Addie Alkhas; Nathan W Wong; Vishal Koparde; Stuart H Yuspa Journal: Oncotarget Date: 2019-12-31