OBJECTIVE: The aim of the study was to determine whether quantitative ultrashort echo time (UTE) -T1ρ magnetic resonance (MR) measurements are sensitive to proteoglycan degradation in human menisci by trypsin digestion. METHODS: Conventional and quantitative UTE-T1ρ MR sequences were performed on 4 meniscal samples using a 3T scanner. Magnetic resonance imaging was performed before and after 4, 8, and 12 hours of trypsin solution immersion, inducing proteoglycan loss. One sample was used as a control. Digest solutions were analyzed for glycosaminoglycan (GAG) content. The UTE-T1ρ studies were analyzed for quantitative changes. RESULTS: Images showed progressive tissue swelling, fiber disorganization, and increase in signal intensity after GAG depletion. The UTE-T1ρ values tended to increase with time after trypsin treatment (P = 0.06). Cumulative GAG loss into the bath showed a trend of increased values for trypsin-treated samples (P = 0.1). CONCLUSIONS: Ultrashort echo time T1ρ measurements can noninvasively detect and quantify severity of meniscal degeneration, which has been correlated with progression of osteoarthritis.
OBJECTIVE: The aim of the study was to determine whether quantitative ultrashort echo time (UTE) -T1ρ magnetic resonance (MR) measurements are sensitive to proteoglycan degradation in human menisci by trypsin digestion. METHODS: Conventional and quantitative UTE-T1ρ MR sequences were performed on 4 meniscal samples using a 3T scanner. Magnetic resonance imaging was performed before and after 4, 8, and 12 hours of trypsin solution immersion, inducing proteoglycan loss. One sample was used as a control. Digest solutions were analyzed for glycosaminoglycan (GAG) content. The UTE-T1ρ studies were analyzed for quantitative changes. RESULTS: Images showed progressive tissue swelling, fiber disorganization, and increase in signal intensity after GAG depletion. The UTE-T1ρ values tended to increase with time after trypsin treatment (P = 0.06). Cumulative GAG loss into the bath showed a trend of increased values for trypsin-treated samples (P = 0.1). CONCLUSIONS: Ultrashort echo time T1ρ measurements can noninvasively detect and quantify severity of meniscal degeneration, which has been correlated with progression of osteoarthritis.
Authors: Won C Bae; Jerry R Dwek; Richard Znamirowski; Sheronda M Statum; Juan C Hermida; Darryl D D'Lima; Robert L Sah; Jiang Du; Christine B Chung Journal: Radiology Date: 2010-03 Impact factor: 11.105
Authors: M-J Berthiaume; J-P Raynauld; J Martel-Pelletier; F Labonté; G Beaudoin; D A Bloch; D Choquette; B Haraoui; R D Altman; M Hochberg; J M Meyer; G A Cline; J-P Pelletier Journal: Ann Rheum Dis Date: 2004-09-16 Impact factor: 19.103
Authors: Ja-Young Choi; Reni Biswas; Won C Bae; Robert Healey; Michael Im; Sheronda Statum; Eric Y Chang; Jiang Du; Graeme M Bydder; Darryl D'Lima; Christine B Chung Journal: Radiology Date: 2016-02-01 Impact factor: 11.105