Jayme R Dowdall1,2,3,4, Peter M Sadow5,3, Christopher Hartnick1,3, Vladimir Vinarsky2,6, Hongmei Mou2, Rui Zhao2, Phillip C Song1,3, Ramon A Franco1,3, Jayaraj Rajagopal1,2,6,7. 1. Department of Otolaryngology, Harvard Medical School. 2. Center for Regenerative Medicine. 3. Massachusetts Eye and Ear Infirmary. 4. Brigham and Women's Hospital, Boston. 5. Departments of Pathology, Massachusetts General Hospital and Harvard Medical School. 6. Department of Internal Medicine, Pulmonary and Critical Care Unit, Massachusetts General Hospital. 7. Harvard Stem Cell Institute, Cambridge, Massachusetts, U.S.A.
Abstract
OBJECTIVES/HYPOTHESIS: A precise molecular schema for classifying the different cell types of the normal human vocal fold epithelium is lacking. We hypothesize that the true vocal fold epithelium has a cellular architecture and organization similar to that of other stratified squamous epithelia including the skin, cornea, oral mucosa, and esophagus. In analogy to disorders of the skin and gastrointestinal tract, a molecular definition of the normal cell types within the human vocal fold epithelium and a description of their geometric relationships should serve as a foundation for characterizing cellular changes associated with metaplasia, dysplasia, and cancer. STUDY DESIGN: Qualitative study with adult human larynges. METHODS: Histologic sections of normal human laryngeal tissue were analyzed for morphology (hematoxylin and eosin) and immunohistochemical protein expression profile, including cytokeratins (CK13 and CK14), cornified envelope proteins (involucrin), basal cells (NGFR/p75), and proliferation markers (Ki67). RESULTS: We demonstrated that three distinct cell strata with unique marker profiles are present within the stratified squamous epithelium of the true vocal fold. We used these definitions to establish that cell proliferation is restricted to certain cell types and layers within the epithelium. These distinct cell types are reproducible across five normal adult larynges. CONCLUSION: We have established that three layers of cells are present within the normal adult stratified squamous epithelium of the true vocal fold. Furthermore, replicating cell populations are largely restricted to the parabasal strata within the epithelium. This delineation of distinct cell populations will facilitate future studies of vocal fold regeneration and cancer. LEVEL OF EVIDENCE: N/A.
OBJECTIVES/HYPOTHESIS: A precise molecular schema for classifying the different cell types of the normal human vocal fold epithelium is lacking. We hypothesize that the true vocal fold epithelium has a cellular architecture and organization similar to that of other stratified squamous epithelia including the skin, cornea, oral mucosa, and esophagus. In analogy to disorders of the skin and gastrointestinal tract, a molecular definition of the normal cell types within the human vocal fold epithelium and a description of their geometric relationships should serve as a foundation for characterizing cellular changes associated with metaplasia, dysplasia, and cancer. STUDY DESIGN: Qualitative study with adult human larynges. METHODS: Histologic sections of normal human laryngeal tissue were analyzed for morphology (hematoxylin and eosin) and immunohistochemical protein expression profile, including cytokeratins (CK13 and CK14), cornified envelope proteins (involucrin), basal cells (NGFR/p75), and proliferation markers (Ki67). RESULTS: We demonstrated that three distinct cell strata with unique marker profiles are present within the stratified squamous epithelium of the true vocal fold. We used these definitions to establish that cell proliferation is restricted to certain cell types and layers within the epithelium. These distinct cell types are reproducible across five normal adult larynges. CONCLUSION: We have established that three layers of cells are present within the normal adult stratified squamous epithelium of the true vocal fold. Furthermore, replicating cell populations are largely restricted to the parabasal strata within the epithelium. This delineation of distinct cell populations will facilitate future studies of vocal fold regeneration and cancer. LEVEL OF EVIDENCE: N/A.
Authors: Carolyn K Novaleski; Bruce D Carter; M Preeti Sivasankar; Sheila H Ridner; Mary S Dietrich; Bernard Rousseau Journal: J Speech Lang Hear Res Date: 2017-05-24 Impact factor: 2.297
Authors: Linqing Li; Jeanna M Stiadle; Hang K Lau; Aidan B Zerdoum; Xinqiao Jia; Susan L Thibeault; Kristi L Kiick Journal: Biomaterials Date: 2016-09-02 Impact factor: 12.479