Literature DB >> 25988525

A transportable, inexpensive electroporator for in utero electroporation.

Torsten Bullmann1,2,3, Thomas Arendt3, Urs Frey1, Carina Hanashima2.   

Abstract

Electroporation is a useful technique to study gene function during development but its broad application is hampered due to the expensive equipment needed. We describe the construction of a transportable, simple and inexpensive electroporator delivering square pulses with varying length and amplitude. The device was successfully used for in utero electroporation in mouse with a performance comparable to that of commercial products.
© 2015 Japanese Society of Developmental Biologists.

Entities:  

Keywords:  cortex; electronic circuit; electroporation; hippocampus

Year:  2015        PMID： 25988525     DOI： 10.1111/dgd.12216

Source DB:  PubMed          Journal:  Dev Growth Differ        ISSN： 0012-1592            Impact factor:   2.053


  7 in total

1.  In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity.

Authors:  Carlos G Briz; Marta Navarrete; José A Esteban; Marta Nieto
Journal:  J Vis Exp       Date:  2017-02-15       Impact factor: 1.355

2.  Modification of a Ready-Made High-Voltage Pulse Generator for Non-Thermal Irreversible Electroporation.

Authors:  K Zhang; J Wang
Journal:  Bull Exp Biol Med       Date:  2022-06-23       Impact factor: 0.804

Review 3.  In Utero Electroporation for Manipulation of Specific Neuronal Populations.

Authors:  Kotaro Yamashiro; Yuji Ikegaya; Nobuyoshi Matsumoto
Journal:  Membranes (Basel)       Date:  2022-05-11

Review 4.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

5.  Increased expression of schizophrenia-associated gene C4 leads to hypoconnectivity of prefrontal cortex and reduced social interaction.

Authors:  Ashley L Comer; Tushare Jinadasa; Balaji Sriram; Rhushikesh A Phadke; Lisa N Kretsge; Thanh P H Nguyen; Giovanna Antognetti; James P Gilbert; Jungjoon Lee; Elena R Newmark; Frances S Hausmann; SaraAnn Rosenthal; Kevin Liu Kot; Yenyu Liu; William W Yen; Borislav Dejanovic; Alberto Cruz-Martín
Journal:  PLoS Biol       Date:  2020-01-14       Impact factor: 8.029

6.  ElectroPen: An ultra-low-cost, electricity-free, portable electroporator.

Authors:  Gaurav Byagathvalli; Soham Sinha; Yan Zhang; Mark P Styczynski; Janet Standeven; M Saad Bhamla
Journal:  PLoS Biol       Date:  2020-01-10       Impact factor: 8.029

7.  A rat model of a focal mosaic expression of PCDH19 replicates human brain developmental abnormalities and behaviours.

Authors:  Andrzej W Cwetsch; Ilias Ziogas; Roberto Narducci; Annalisa Savardi; Maria Bolla; Bruno Pinto; Laura E Perlini; Silvia Bassani; Maria Passafaro; Laura Cancedda
Journal:  Brain Commun       Date:  2022-04-05
  7 in total

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