T M Parisotto1, R Stipp2, L K A Rodrigues3, R O Mattos-Graner2, L S Costa4, M Nobre-Dos-Santos5. 1. Laboratory of Microbiology and Molecular Biology, Sao Francisco University Dental School, Bragança Paulista, SP, Brazil. 2. Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Brazil. 3. Department of Operative Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil. 4. Federal University of Rio de Janeiro State, Rio de Janeiro, RJ, Brazil. 5. Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas, Brazil. Electronic address: nobre@fop.unicamp.br.
Abstract
BACKGROUND: Insoluble polysaccharide (IP) has been associated with caries prevalence in young children. However, the power of IP to predict ECC needs to be demonstrated. AIMS: To assess the relationships between early childhood caries (ECC) and extracellular insoluble polysaccharides (IP) in dental plaque, sugar exposure and cariogenic microorganisms. DESIGN: Visible plaque on maxillary incisors was recorded, followed by caries diagnosis in 65 preschoolers (3-4 years) at baseline and after 1 year. Plaque was collected for mutans streptococci (MS), total microorganism (TM) and lactobacilli (LB) enumerations in selective media, as well as for IP analysis, which was later assessed by colorimetry. Sugar/sucrose exposure was assessed by a diet chart. RESULTS: Positive correlations were found among the prevalence of caries and MS, TM, LB, solid sucrose and visible dental plaque. Additionally, children with IP concentrations in dental plaque higher than 2.36 μg/mg (odds ratio-OR=6.8), with visible plaque on maxillary incisors (OR=4.3), harbouring LB (OR=13) and exposed to solid sugar more than twice/day (OR=5) showed higher risk of developing caries (p<0.05). CONCLUSION: Extracellular insoluble polysaccharides, solid sugar/sucrose, visible dental plaque and cariogenic microorganisms could predict caries development, partially explaining the ECC pattern.
BACKGROUND: Insoluble polysaccharide (IP) has been associated with caries prevalence in young children. However, the power of IP to predict ECC needs to be demonstrated. AIMS: To assess the relationships between early childhood caries (ECC) and extracellular insoluble polysaccharides (IP) in dental plaque, sugar exposure and cariogenic microorganisms. DESIGN: Visible plaque on maxillary incisors was recorded, followed by caries diagnosis in 65 preschoolers (3-4 years) at baseline and after 1 year. Plaque was collected for mutans streptococci (MS), total microorganism (TM) and lactobacilli (LB) enumerations in selective media, as well as for IP analysis, which was later assessed by colorimetry. Sugar/sucrose exposure was assessed by a diet chart. RESULTS: Positive correlations were found among the prevalence of caries and MS, TM, LB, solid sucrose and visible dental plaque. Additionally, children with IP concentrations in dental plaque higher than 2.36 μg/mg (odds ratio-OR=6.8), with visible plaque on maxillary incisors (OR=4.3), harbouring LB (OR=13) and exposed to solid sugar more than twice/day (OR=5) showed higher risk of developing caries (p<0.05). CONCLUSION: Extracellular insoluble polysaccharides, solid sugar/sucrose, visible dental plaque and cariogenic microorganisms could predict caries development, partially explaining the ECC pattern.
Authors: Lídia Audrey Rocha Valadas; Patrícia Leal Dantas Lobo; Said Gonçalves da Cruz Fonseca; Francisco Vagnaldo Fechine; Edilson Martins Rodrigues Neto; Marta Maria de França Fonteles; Lorena Ribeiro de Aguiar Trévia; Hilda Lara Prado Vasconcelos; Sandra Mara da Silva Lima; Mara Assef Leitao Lotif; Analice Mendes Barreto Fernandes; Mary Anne Medeiros Bandeira Journal: Evid Based Complement Alternat Med Date: 2021-03-25 Impact factor: 2.629