| Literature DB >> 25981484 |
Scott J Gratz1, Melissa M Harrison, Jill Wildonger, Kate M O'Connor-Giles.
Abstract
The readily programmable CRISPR-Cas9 system is transforming genome engineering. We and others have adapted the S. pyogenes CRISPR-Cas9 system to precisely engineer the Drosophila genome and demonstrated that these modifications are efficiently transmitted through the germline. Here we provide a detailed protocol for engineering small indels, defined deletions, and targeted insertion of exogenous DNA sequences within one month using a rapid DNA injection-based approach.Entities:
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Year: 2015 PMID： 25981484 PMCID： PMC4467458 DOI： 10.1007/978-1-4939-2687-9_22
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745