Siyuan Li1, Zhuoyu Gu1, Zhiwei Xiao1, Ting Zhou2, Jun Li3, Kan Sun3. 1. Medical College of Shihezi University, Key Laboratory of Ministry of Education, Xinjiang Endemic and Ethnic Diseases Shihezi 832002, China. 2. Key Laboratory, First Affiliated Hospital, Medical College of Shihezi University Shihezi 832002, China. 3. Department of Endocrinology, First Affiliated Hospital, Medical College of Shihezi University Shihezi 832002, China.
Abstract
OBJECTIVE: To investigate whether celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, can attenuate proliferation, migration, invasion and MMP-14 expression in pancreatic cancer cells PANC-1 and the possible anti-tumor mechanism of celecoxib. METHODS: Human pancreatic cancer cell line PANC-1 cells were treated with diverse concentrations of celecoxib (20, 60, 100 μmol/L). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, transwell invasion assay, and scratch assay separately. At the same time, the protein expression of COX-2 and MMP-14 was assessed by ELISA. RESULTS: The capabilities of proliferation, invasion and migration in PANC-1 cells were attenuated in a concentration-dependent manner after treated with celecoxib, followed by the down-regulation of the protein expression of COX-2 and MMP-14. In addition, MMP-14 expression was significantly positively correlated with COX-2 expression. CONCLUSIONS: COX-2 inhibitor celecoxib can inhibit the proliferation, invasion and migration of PANC-1 cells via down-regulating the expression of MMP-14 in a concentration-dependent manner, thus contributing to its anti-tumor effect in pancreatic cancer.
OBJECTIVE: To investigate whether celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, can attenuate proliferation, migration, invasion and MMP-14 expression in pancreatic cancer cells PANC-1 and the possible anti-tumor mechanism of celecoxib. METHODS:Humanpancreatic cancer cell line PANC-1 cells were treated with diverse concentrations of celecoxib (20, 60, 100 μmol/L). Cell proliferation, invasion and migration capabilities were measured by MTT colorimetry, transwell invasion assay, and scratch assay separately. At the same time, the protein expression of COX-2 and MMP-14 was assessed by ELISA. RESULTS: The capabilities of proliferation, invasion and migration in PANC-1 cells were attenuated in a concentration-dependent manner after treated with celecoxib, followed by the down-regulation of the protein expression of COX-2 and MMP-14. In addition, MMP-14 expression was significantly positively correlated with COX-2 expression. CONCLUSIONS:COX-2 inhibitor celecoxib can inhibit the proliferation, invasion and migration of PANC-1 cells via down-regulating the expression of MMP-14 in a concentration-dependent manner, thus contributing to its anti-tumor effect in pancreatic cancer.
Authors: Ning Ding; Xiao-Xing Cui; Zhi Gao; Huarong Huang; Xingchuan Wei; Zhiyun Du; Yong Lin; Weichung Joe Shih; Arnold B Rabson; Allan H Conney; Chunhong Hu; Xi Zheng Journal: Int J Oncol Date: 2014-03-19 Impact factor: 5.650