| Literature DB >> 25973059 |
Wen Yan1, Chengluan Xuan2, Lei Xuan3, Rihao Xu4, Junnan Wang5.
Abstract
The aim of this study was to assess the role of platelet activating factor (PAF) antagonist BN52021 in doxorubicin induced cardiotoxicity and to explore the mechanisms. H9c2 cardiomyocytes were employed to investigate the effect of BN52021 on doxorubicin induced cell viability and cell apoptosis. Signaling pathway of caspase 3, cytochrome c, calcium and p38 mitogen-activated protein (MAPK) was determined during the doxorubicin induced apoptosis. Our results showed BN52021 pretreatment could protected cell death induced by doxorubicin in H9c2 cardiomyocytes. Decrease concentration of [Ca(2+)] and expression of phosphorylated P38 MAPK were accounted for the protection effect. Inhibition of signaling pathway of calcium and p38 MAPK showed similar effect exerted by BN52021 in doxorubicin induced cell apoptosis. Our results demonstrated BN52021 protected against doxorubicin induced cell death in H9c2 cardiomyocytes by calcium and p38 MAPK signaling in vitro. These finding may give insight on the treatment of doxorubicin induced cardiomyopathy.Entities:
Keywords: BN52021; MAPK signaling; calcium signaling; cardiotoxicity; doxorubicin
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Year: 2015 PMID： 25973059 PMCID： PMC4396248
Source DB: PubMed Journal: Int J Clin Exp Pathol ISSN： 1936-2625