Carly E Visentin1, Shannon Masih1, Lesley Plumptre1, Olga Malysheva2, Daiva E Nielsen3, Kyoung-Jin Sohn4, Anna Ly4, Andrea Y Lausman5, Howard Berger5, Ruth Croxford6, Ahmed El-Sohemy3, Marie A Caudill2, Deborah L O'Connor7, Young-In Kim8. 1. Department of Nutritional Sciences, University of Toronto, Toronto, Canada; Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, Canada; 2. Division of Nutritional Sciences, College of Human Ecology, Cornell University, Ithaca, NY; and. 3. Department of Nutritional Sciences, University of Toronto, Toronto, Canada; 4. Departments of Medicine and Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, Canada; 5. Obstetrics and Gynecology, St. Michael's Hospital and University of Toronto, Toronto, Canada; 6. freelance statistics consultant, Toronto, Canada. 7. Department of Nutritional Sciences, University of Toronto, Toronto, Canada; Research Institute, the Hospital for Sick Children, Toronto, Canada; 8. Department of Nutritional Sciences, University of Toronto, Toronto, Canada; Departments of Medicine and Keenan Research Centre for Biomedical Science, St. Michael's Hospital, Toronto, Canada; youngin.kim@utoronto.ca.
Abstract
BACKGROUND: Choline deficiency during pregnancy can lead to adverse birth outcomes, including impaired neurodevelopment and birth defects. Genetic variants of choline and one-carbon metabolism may also influence birth outcomes by altering plasma choline concentrations. The effects of maternal ad libitum choline intake during pregnancy and fetal genetic variants on maternal and cord concentrations of choline and its metabolites are unknown. OBJECTIVES: This prospective study sought to assess the effect of 1) maternal dietary choline intake on maternal and cord plasma concentrations of choline and its metabolites, and 2) fetal genetic polymorphisms on cord plasma concentrations. METHODS: The dietary choline intake of 368 pregnant Canadian women was assessed in early (0-16 wk) and late (23-37 wk) pregnancy with the use of a food frequency questionnaire. Plasma concentrations of free choline and its metabolites were measured in maternal samples at recruitment and delivery, and in the cord blood. Ten fetal genetic variants in choline and one-carbon metabolism were assessed for their association with cord plasma concentrations of free choline and its metabolites. RESULTS: Mean maternal plasma free choline, dimethylglycine, and trimethylamine N-oxide (TMAO) concentrations increased during pregnancy by 49%, 17%, and 13%, respectively (P < 0.005), whereas betaine concentrations decreased by 21% (P < 0.005). Cord plasma concentrations of free choline, betaine, dimethylglycine, and TMAO were 3.2, 2.0, 1.3, and 0.88 times corresponding maternal concentrations at delivery, respectively (all P < 0.005). Maternal plasma concentrations of betaine, dimethylglycine, and TMAO (r(2) = 0.19-0.51; P < 0.0001) at delivery were moderately strong, whereas maternal concentrations of free choline were not significant (r(2) = 0.12; P = 0.06), predictors of cord plasma concentrations of these metabolites. Neither maternal dietary intake nor fetal genetic variants predicted maternal or cord plasma concentrations of choline and its metabolites. CONCLUSION: These data collectively indicate that maternal choline status, but not fetal genotype, influences cord plasma concentrations of choline metabolites. This trial was registered at clinicaltrials.gov as NCT02244684.
BACKGROUND:Cholinedeficiency during pregnancy can lead to adverse birth outcomes, including impaired neurodevelopment and birth defects. Genetic variants of choline and one-carbon metabolism may also influence birth outcomes by altering plasma choline concentrations. The effects of maternal ad libitum choline intake during pregnancy and fetal genetic variants on maternal and cord concentrations of choline and its metabolites are unknown. OBJECTIVES: This prospective study sought to assess the effect of 1) maternal dietary choline intake on maternal and cord plasma concentrations of choline and its metabolites, and 2) fetal genetic polymorphisms on cord plasma concentrations. METHODS: The dietary choline intake of 368 pregnant Canadian women was assessed in early (0-16 wk) and late (23-37 wk) pregnancy with the use of a food frequency questionnaire. Plasma concentrations of free choline and its metabolites were measured in maternal samples at recruitment and delivery, and in the cord blood. Ten fetal genetic variants in choline and one-carbon metabolism were assessed for their association with cord plasma concentrations of free choline and its metabolites. RESULTS: Mean maternal plasma free choline, dimethylglycine, and trimethylamine N-oxide (TMAO) concentrations increased during pregnancy by 49%, 17%, and 13%, respectively (P < 0.005), whereas betaine concentrations decreased by 21% (P < 0.005). Cord plasma concentrations of free choline, betaine, dimethylglycine, and TMAO were 3.2, 2.0, 1.3, and 0.88 times corresponding maternal concentrations at delivery, respectively (all P < 0.005). Maternal plasma concentrations of betaine, dimethylglycine, and TMAO (r(2) = 0.19-0.51; P < 0.0001) at delivery were moderately strong, whereas maternal concentrations of free choline were not significant (r(2) = 0.12; P = 0.06), predictors of cord plasma concentrations of these metabolites. Neither maternal dietary intake nor fetal genetic variants predicted maternal or cord plasma concentrations of choline and its metabolites. CONCLUSION: These data collectively indicate that maternal choline status, but not fetal genotype, influences cord plasma concentrations of choline metabolites. This trial was registered at clinicaltrials.gov as NCT02244684.
Authors: John T Brosnan; Lesley Plumptre; Margaret E Brosnan; Theerawat Pongnopparat; Shannon P Masih; Carly E Visentin; Howard Berger; Yvonne Lamers; Marie A Caudill; Olga V Malysheva; Deborah L O'Connor; Young-In Kim Journal: Am J Clin Nutr Date: 2019-11-01 Impact factor: 7.045
Authors: Carolyn F McCabe; Jennifer L LaBarre; Steven E Domino; Marjorie C Treadwell; Ana Baylin; Charles F Burant; Dana C Dolinoy; Vasantha Padmanabhan; Jaclyn M Goodrich Journal: J Nutr Biochem Date: 2022-01-10 Impact factor: 6.048
Authors: Linde van Lee; Sarah R Crozier; Izzuddin M Aris; Mya T Tint; Suresh Anand Sadananthan; Navin Michael; Phaik Ling Quah; Sian M Robinson; Hazel M Inskip; Nicholas C Harvey; Mary Barker; Cyrus Cooper; Sendhil S Velan; Yung Seng Lee; Marielle V Fortier; Fabian Yap; Peter D Gluckman; Kok Hian Tan; Lynette P Shek; Yap-Seng Chong; Keith M Godfrey; Mary F F Chong Journal: Int J Epidemiol Date: 2019-04-01 Impact factor: 7.196
Authors: Anna K Knight; Hea Jin Park; Dorothy B Hausman; Jennifer M Fleming; Victoria L Bland; Gisselle Rosa; Elizabeth M Kennedy; Marie A Caudill; Olga Malysheva; Gail P A Kauwell; Andrew Sokolow; Susan Fisher; Alicia K Smith; Lynn B Bailey Journal: Sci Rep Date: 2018-11-15 Impact factor: 4.379
Authors: Tiina Jääskeläinen; Olli Kärkkäinen; Jenna Jokkala; Kaisa Litonius; Seppo Heinonen; Seppo Auriola; Marko Lehtonen; Kati Hanhineva; Hannele Laivuori Journal: Sci Rep Date: 2018-10-02 Impact factor: 4.379