Gaurav Sharma1, Ming Tao1, Kui Ding1, David Yu1, William King1, Galina Deyneko1, Xiaosong Wang1, Alban Longchamp1, Frederick J Schoen1, C Keith Ozaki1, Marcus E Semel2. 1. From the Departments of Surgery (G.S., M.T., K.D., W.K., A.L., C.K.O., M.E.S.) and Pathology (F.J.S.), Brigham and Women's Hospital/Harvard Medical School, Boston, MA; University of Washington School of Medicine, Seattle, WA (D.Y.); and Novartis Institutes for BioMedical Research, Cambridge, MA (G.D., X.W.). 2. From the Departments of Surgery (G.S., M.T., K.D., W.K., A.L., C.K.O., M.E.S.) and Pathology (F.J.S.), Brigham and Women's Hospital/Harvard Medical School, Boston, MA; University of Washington School of Medicine, Seattle, WA (D.Y.); and Novartis Institutes for BioMedical Research, Cambridge, MA (G.D., X.W.). msemel1@partners.org.
Abstract
BACKGROUND AND PURPOSE: Recent symptoms stand as a major determinant of stroke risk in patients with carotid stenosis, likely reflective of atherosclerotic plaque destabilization. In view of emerging links between vascular and adipose biology, we hypothesized that human perivascular adipose characteristics associate with carotid disease symptom status. METHODS:Clinical history, carotid plaques, blood, and subcutaneous and perivascular adipose tissues were prospectively collected from patients undergoing carotid endarterectomy. Nine adipose-associated biological mediators were assayed and compared in patients with symptomatic (n=15) versus asymptomatic (n=19) disease. Bonferroni correction was performed for multiple testing (α/9=0.006). RESULTS: Symptomatic patients had 1.9-fold higher perivascular adiponectin levels (P=0.005). Other circulating, subcutaneous, and perivascular biomarkers, as well as microscopic plaque characteristics, did not differ between symptomatic and asymptomatic patients. CONCLUSIONS: Symptomatic and asymptomatic carotid endarterectomy patients display a tissue-specific difference in perivascular adipose adiponectin. This difference, which was not seen in plasma or subcutaneous compartments, supports a potential local paracrine relationship with vascular disease processes that may be related to stroke mechanisms.
RCT Entities:
BACKGROUND AND PURPOSE: Recent symptoms stand as a major determinant of stroke risk in patients with carotid stenosis, likely reflective of atherosclerotic plaque destabilization. In view of emerging links between vascular and adipose biology, we hypothesized that human perivascular adipose characteristics associate with carotid disease symptom status. METHODS: Clinical history, carotid plaques, blood, and subcutaneous and perivascular adipose tissues were prospectively collected from patients undergoing carotid endarterectomy. Nine adipose-associated biological mediators were assayed and compared in patients with symptomatic (n=15) versus asymptomatic (n=19) disease. Bonferroni correction was performed for multiple testing (α/9=0.006). RESULTS: Symptomatic patients had 1.9-fold higher perivascular adiponectin levels (P=0.005). Other circulating, subcutaneous, and perivascular biomarkers, as well as microscopic plaque characteristics, did not differ between symptomatic and asymptomatic patients. CONCLUSIONS: Symptomatic and asymptomatic carotid endarterectomy patients display a tissue-specific difference in perivascular adipose adiponectin. This difference, which was not seen in plasma or subcutaneous compartments, supports a potential local paracrine relationship with vascular disease processes that may be related to stroke mechanisms.
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