Gongjian Zhu1,2, Lan Wang1, Hongyun Guo1, Lingeng Lu3, Suisheng Yang1, Tao Wang1, Huan Guo1, Haitao Wang1, Jianping Min1, Kai Yang1, Xuezhong Chen1, Yuanqiang Liu1, Zhiping Wang4, Haixiang Su1. 1. 1 Gansu Provincial Academy of Medical Sciences, Gansu Provincial Cancer Hospital , Lanzhou, People's Republic of China . 2. 2 School of Life Sciences, Lanzhou University , Lanzhou, China . 3. 3 Department of Chronic Disease Epidemiology, School of Public Health, School of Medicine, Yale Cancer Center, Yale University , New Haven, Connecticut. 4. 4 Institute of Urology, The Second Hospital of Lanzhou University , Lanzhou, China .
Abstract
AIMS: Polymorphisms in DNA damage repair genes may affect DNA repair capacity and modulate breast cancer susceptibility. In this study, we aimed to analyze two polymorphisms for each of the DNA repair genes X-ray repair cross-complementing group 1 (XRCC1) rs25487 and rs1799782 and excision repair cross-complementing group 1 (ERCC1) rs3212964 and rs11615, to evaluate their associations with the risk of sporadic breast cancer in Han women in the Gansu Province of China. METHODS: Genotypes were determined by a polymerase chain reaction-based approach for 101 patients with breast cancer and in 101 disease-free controls. RESULTS: We found that individuals with the AA genotype at XRCC1 rs25487 had a significantly increased risk of breast cancer compared with GG genotype (p<0.001, odds ratio [OR]=6.39, 95% confidence interval [CI]: 2.18-18.65). The dominant model showed that the combined rs25487 genotypes (AA+AG) increased the disease risk (p<0.001, OR=3.17, 95% CI: 1.76-5.72). However, no statistical associations were found between rs1799782 in XRCC1, or rs3212964 and rs11615 in ERCC1 and the risk of disease. In haplotype analysis, the GC haplotype in XRCC1 conferred an increased risk (p<0.001) with a 4.78-fold increase for each copy (95% CI: 2.52-8.72). Significant associations were also shown between the single nucleotide polymorphisms (SNPs) and the status of estrogen receptor (ER), progesterone receptor (PR), and HER-2. CONCLUSIONS: The results suggest that the XRCC1 rs25487 polymorphism may increase the risk of breast cancer.
AIMS: Polymorphisms in DNA damage repair genes may affect DNA repair capacity and modulate breast cancer susceptibility. In this study, we aimed to analyze two polymorphisms for each of the DNA repair genes X-ray repair cross-complementing group 1 (XRCC1) rs25487 and rs1799782 and excision repair cross-complementing group 1 (ERCC1) rs3212964 and rs11615, to evaluate their associations with the risk of sporadic breast cancer in Han women in the Gansu Province of China. METHODS: Genotypes were determined by a polymerase chain reaction-based approach for 101 patients with breast cancer and in 101 disease-free controls. RESULTS: We found that individuals with the AA genotype at XRCC1rs25487 had a significantly increased risk of breast cancer compared with GG genotype (p<0.001, odds ratio [OR]=6.39, 95% confidence interval [CI]: 2.18-18.65). The dominant model showed that the combined rs25487 genotypes (AA+AG) increased the disease risk (p<0.001, OR=3.17, 95% CI: 1.76-5.72). However, no statistical associations were found between rs1799782 in XRCC1, or rs3212964 and rs11615 in ERCC1 and the risk of disease. In haplotype analysis, the GC haplotype in XRCC1 conferred an increased risk (p<0.001) with a 4.78-fold increase for each copy (95% CI: 2.52-8.72). Significant associations were also shown between the single nucleotide polymorphisms (SNPs) and the status of estrogen receptor (ER), progesterone receptor (PR), and HER-2. CONCLUSIONS: The results suggest that the XRCC1rs25487 polymorphism may increase the risk of breast cancer.
Authors: Bang-Shun He; Tao Xu; Yu-Qin Pan; Han-Jin Wang; William C Cho; Kang Lin; Hui-Ling Sun; Tian-Yi Gao; Shu-Kui Wang Journal: Oncotarget Date: 2016-12-20