Diana Nogueira1, Susana Rocha2, Estela Abreu3, Elísio Costa2, Alice Santos-Silva2. 1. Serviço de Patologia Clínica, Laboratório de Hematologia, Centro Hospitalar S. João, Porto, Portugal. 2. Departamento de Ciências Biológicas, Laboratório de Bioquímica, Faculdade Farmácia, Universidade do Porto, Porto, Portugal ; UCIBIO @ REQUIMTEUniversidade do Porto, Porto, Portugal. 3. Serviço de Imunohemoterapia, Centro Hospitalar S. João, Porto, Portugal.
Abstract
BACKGROUND: To evaluate biochemical and cellular changes associated with the storage of leukocyte-depleted red blood cells (RBCs). METHODS: We investigated 10 leukocyte-depleted RBC units, randomly chosen from volunteer donors. Every week an aliquot was collected for laboratorial evaluation, which included complete cell blood count, glucose-6-phosphate dehydrogenase (G6PD) activity, extracellular sodium, potassium and pH, membrane-bound hemoglobin (MBH), band 3 profile, and quantification of RBC membrane proteins composition. RESULTS: We observed an increase in mean cell volume (from 91.86 ± 4.65 fl to 98.10 ± 5.80 fl, day 0 vs. day 21; p < 0.05), red cell distribution width, percentage of macrocytic RBCs, reticulocyte hemoglobin content and a decreased percentage of microcytic RBCs, mean cell volume concentration and G6PD activity. The extracellular concentration of sodium decreased, and that of potassium increased significantly over time. RBC membrane composition revealed an increase in spectrin/ankyrin ratio after 21 days (from 4.84 ± 0.99 to 5.27 ± 0.94, day 0 vs. day 21; p < 0.05). At day 35, a decrease in ankyrin (from 6.44 ± 1.70% to 5.49 ± 1.96%, day 0 vs. day 35; p < 0.05), in protein 4.1/band 3, protein 4.2/band 3, and ankyrin/band 3 ratios and in band 5 was observed. CONCLUSIONS: Our data show that leukocyte-depleted RBCs present changes in the RBC morphology, membrane protein composition, enzymatic activity, and extracellular electrolyte concentration and pH.
BACKGROUND: To evaluate biochemical and cellular changes associated with the storage of leukocyte-depleted red blood cells (RBCs). METHODS: We investigated 10 leukocyte-depleted RBC units, randomly chosen from volunteer donors. Every week an aliquot was collected for laboratorial evaluation, which included complete cell blood count, glucose-6-phosphate dehydrogenase (G6PD) activity, extracellular sodium, potassium and pH, membrane-bound hemoglobin (MBH), band 3 profile, and quantification of RBC membrane proteins composition. RESULTS: We observed an increase in mean cell volume (from 91.86 ± 4.65 fl to 98.10 ± 5.80 fl, day 0 vs. day 21; p < 0.05), red cell distribution width, percentage of macrocytic RBCs, reticulocyte hemoglobin content and a decreased percentage of microcytic RBCs, mean cell volume concentration and G6PD activity. The extracellular concentration of sodium decreased, and that of potassium increased significantly over time. RBC membrane composition revealed an increase in spectrin/ankyrin ratio after 21 days (from 4.84 ± 0.99 to 5.27 ± 0.94, day 0 vs. day 21; p < 0.05). At day 35, a decrease in ankyrin (from 6.44 ± 1.70% to 5.49 ± 1.96%, day 0 vs. day 35; p < 0.05), in protein 4.1/band 3, protein 4.2/band 3, and ankyrin/band 3 ratios and in band 5 was observed. CONCLUSIONS: Our data show that leukocyte-depleted RBCs present changes in the RBC morphology, membrane protein composition, enzymatic activity, and extracellular electrolyte concentration and pH.
Entities:
Keywords:
Leukocyte depletion; Membrane proteins; RBC; Red cell aging; Transfusion
Authors: Eldad A Hod; Ning Zhang; Set A Sokol; Boguslaw S Wojczyk; Richard O Francis; Daniel Ansaldi; Kevin P Francis; Phyllis Della-Latta; Susan Whittier; Sujit Sheth; Jeanne E Hendrickson; James C Zimring; Gary M Brittenham; Steven L Spitalnik Journal: Blood Date: 2010-03-18 Impact factor: 22.113
Authors: Marianna H Antonelou; Anastasios G Kriebardis; Konstantinos E Stamoulis; Effrosini Economou-Petersen; Lukas H Margaritis; Issidora S Papassideri Journal: Transfusion Date: 2009-10-23 Impact factor: 3.157
Authors: Rajeev K Mehlotra; D'Arbra Blankenship; Rosalind E Howes; Tovonahary A Rakotomanga; Brune Ramiranirina; Stephanie Ramboarina; Thierry Franchard; Marlin H Linger; Melinda Zikursh-Blood; Arsène C Ratsimbasoa; Peter A Zimmerman; Brian T Grimberg Journal: Malar J Date: 2017-11-03 Impact factor: 2.979
Authors: Anna Zimna; Magdalena Kaczmarska; Ewa Szczesny-Malysiak; Aleksandra Wajda; Katarzyna Bulat; Fatih Celal Alcicek; Malgorzata Zygmunt; Tomasz Sacha; Katarzyna Maria Marzec Journal: Int J Mol Sci Date: 2021-03-12 Impact factor: 5.923