Literature DB >> 25957458

Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii.

Marie-Pierre Brenier-Pinchart1, Elodie Capderou2, Rose-Laurence Bertini2, Sébastien Bailly2, Hélène Fricker-Hidalgo2, Emmanuelle Varlet-Marie3, Jean-Benjamin Murat2, Yvon Sterkers3, Fériel Touafek4, Patrick Bastien3, Hervé Pelloux5.   

Abstract

Early detection of Toxoplasma tachyzoites circulating in blood using PCR is recommended for immunosuppressed patients at high risk for disseminated toxoplasmosis. Using a toxoplasmosis mouse model, we show that the sensitivity of detection is higher using buffy coat isolated from a large blood volume than using whole blood for this molecular monitoring.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Buffy coat; Diagnosis; PCR; Toxoplasma gondii; Toxoplasmosis; Whole blood

Mesh：

Year:  2015        PMID： 25957458     DOI： 10.1016/j.diagmicrobio.2015.04.004

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  5 in total

1.  PCR-based diagnosis is not always useful in the acute acquired toxoplasmosis in immunocompetent individuals.

Authors:  Elizabeth Souza Neves; Otavio Melo Espíndola; André Curi; Maria Regina Amendoeira; Danielle Nascimento Rocha; Leonardo Henrique Ferreira Gomes; Letícia Cunha Guida
Journal:  Parasitol Res       Date:  2021-01-08       Impact factor: 2.289

2.  Sero-molecular survey on Toxoplasma gondii infection among drug addicted and non-addicted individuals: a case-control study.

Authors:  Majid Sharifzadeh; Hassan Rezanezhad; Kavous Solhjoo; Zahra Kargar Jahromi; Enayatollah Shadmand; Saeed Shahabi; Ali Taghipour
Journal:  BMC Infect Dis       Date:  2022-01-04       Impact factor: 3.090

3.  Performance Testing of PCR Assay in Blood Samples for the Diagnosis of Toxoplasmic Encephalitis in AIDS Patients from the French Departments of America and Genetic Diversity of Toxoplasma gondii: A Prospective and Multicentric Study.

Authors:  Daniel Ajzenberg; Isabelle Lamaury; Magalie Demar; Cyrille Vautrin; André Cabié; Stéphane Simon; Muriel Nicolas; Nicole Desbois-Nogard; Rachida Boukhari; Homayoun Riahi; Marie-Laure Dardé; Patrice Massip; Michel Dupon; Pierre-Marie Preux; Anaïs Labrunie; Marie-Paule Boncoeur
Journal:  PLoS Negl Trop Dis       Date:  2016-06-29

4.  Serum IgG Anti-Toxoplasma gondii Antibody Concentrations Do Not Correlate Nested PCR Results in Blood Donors.

Authors:  Fabiana Nakashima; Valquíria Sousa Pardo; Marcos Paulo Miola; Fernando Henrique Antunes Murata; Natalia Paduan; Stefani Miqueline Longo; Cinara Cássia Brandão de Mattos; Vera Lucia Pereira-Chioccola; Octávio Ricci; Luiz Carlos de Mattos
Journal:  Front Cell Infect Microbiol       Date:  2020-01-14       Impact factor: 5.293

5.  Impact of pre-analytic step duration on molecular diagnosis of toxoplasmosis for five types of biological samples.

Authors:  Marie-Pierre Brenier-Pinchart; Emmanuelle Varlet-Marie; Florence Robert-Gangneux; Denis Filisetti; Juliette Guitard; Yvon Sterkers; Hélène Yera; Hervé Pelloux; Patrick Bastien
Journal:  PLoS One       Date:  2021-02-17       Impact factor: 3.240
  5 in total

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