| Literature DB >> 25953398 |
B Bhaskar Gollapudi1, Anthony M Lynch2, Robert H Heflich3, Stephen D Dertinger4, Vasily N Dobrovolsky5, Roland Froetschl6, Katsuyoshi Horibata7, Michelle O Kenyon8, Takafumi Kimoto9, David P Lovell10, Leon F Stankowski11, Paul A White12, Kristine L Witt13, Jennifer Y Tanir14.
Abstract
The in vivo Pig-a assay uses flow cytometry to measure phenotypic variants for antibody binding to cell surface glycosylphosphatidylinositol (GPI)-anchored proteins. There is good evidence suggesting that the absence of antibody binding is the result of a mutation in the endogenous X-linked Pig-a gene, which forms the rationale for the assay. Although the assay has been performed with several types of hematopoietic cells and in a variety of mammalian species, including humans, currently it is optimized only for measuring CD59-deficient (presumed Pig-a mutant) erythrocytes in the peripheral blood of rats. An expert workgroup formed by the International Workshop on Genotoxicity Testing considered the state of assay development and the potential of the assay for regulatory use. Consensus was reached on what is known about the Pig-a assay and how it should be conducted, and recommendations were made on additional data and refinements that would help to further enhance the assay for use in hazard identification and risk assessment. Published by Elsevier B.V.Entities:
Keywords: CD59; Flow cytometry; Glycosylphosphatidylinositol; Mutation; Red blood cells; Reticulocytes
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Year: 2014 PMID： 25953398 DOI： 10.1016/j.mrgentox.2014.09.007
Source DB: PubMed Journal: Mutat Res Genet Toxicol Environ Mutagen ISSN： 1383-5718 Impact factor: 2.873