Draft Genome Sequence of Paenibacillus polymyxa EBL06, a Plant Growth-Promoting Bacterium Isolated from Wheat Phyllosphere.

Paenibacillus polymyxa strain EBL06 is a plant growth-promoting bacterium with high antifungal activity. The estimated genome of this strain is 5.68 Mb in size and harbors 4,792 coding sequences (CDSs).

GENOME ANNOUNCEMENT

Paenibacillus polymyxa (formally Bacillus polymyxa) is a Gram-positive, endospore-forming bacterium belonging to a diverse group of plant growth-promoting bacteria (PGPB). Our previous study showed that P. polymyxa strain EBL06, isolated from wheat phyllosphere, had a high level of antagonistic fungus activity (1) and could stimulate tea plant growth and improve the quality of Brassica rapa L. chinensis (2, 3).

Here, we report the draft genome sequence of P. polymyxa EBL06. Genome DNA was extracted and sequenced using the Illumina HiSeq 2000 platform. Shotgun sequencing produced 17,775,760 paired-end reads with approximately 264-fold coverage. Filtered reads were assembled filled by SOAPdenovo 2.04 (4). This assembly generated 102 contigs, with an N50 length of 627,482 bp and an average length of 55,594 bp, which were assembled into 45 scaffolds, with an N50 length of 1,481,754 bp and an average length of 126,218 bp. Genome annotation was performed by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) (http://www.ncbi.nlm.nih.gov/genome/annotation_prok/).

The draft genome is 5.68 Mb, with a G+C% content of 45.59%. A total of 4,792 coding sequences (CDSs), 105 pseudogenes, 41 tRNA genes, 1 noncoding RNA (ncRNA), and 9 rRNAs were identified.

Average nucleotide identity (ANI) analysis (5) revealed that P. polymyxa EBL06 is phylogenetically related to P. polymyxa SC2 (94.5%) (6), P. polymyxa M1 (94.5%) (7), P. polymyxa E681 (89.4%) (8), P. polymyxa SQR-21 (98.6%) (9), P. polymyxa CR1 (89.6%) (10), and P. polymyxa Sb3-1 (94.7%) (11).

Nucleotide sequence accession number.

The whole-genome shotgun project of P. polymyxa EBL06 has been deposited at DDBI/EMBL/GenBank under the accession no. JYCW00000000. The version described in this paper is the first version.