| Literature DB >> 25951414 |
Xiuxin Luo1, Yongli Guo1, Jun Bao2, Ying Liu1, Dong An1, Bo Ma1, Mingchun Gao3, Junwei Wang4.
Abstract
A novel bovine interferon-ω (BoIFN-ω) gene, which encodes a protein of 195 amino acids with a 23-amino acid signal peptide, was amplified from bovine liver genomic DNA through PCR and named BoIFN-ω24 according to its position in the bovine genome. In this study, the recombinant protein was expressed in Escherichia coli and antiviral or antiproliferation activity was determined in vitro. Results showed that BoIFN-ω24 exhibits high antiviral activity, which can be abrogated using PAb against BoIFN-ω24, and inhibits cell proliferation. BoIFN-ω24 also presents high sensitivity to trypsin and stability at pH 2.0 or 65°C, which are typical characteristics of type I IFN. This study revealed that BoIFN-ω24 is a potential novel effective therapeutic agent and provided a basis for further research on the BoIFN-ω multigene family.Entities:
Keywords: Antiproliferation activity; Antiviral activity; Bovine; IFN-ω24; Physicochemical characteristics
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Year: 2015 PMID： 25951414 DOI： 10.1016/j.molimm.2015.04.009
Source DB: PubMed Journal: Mol Immunol ISSN： 0161-5890 Impact factor: 4.407