Literature DB >> 25950972

Solution NMR Structure Determination of Polytopic α-Helical Membrane Proteins: A Guide to Spin Label Paramagnetic Relaxation Enhancement Restraints.

Linda Columbus1, Brett Kroncke2.   

Abstract

Solution nuclear magnetic resonance structures of polytopic α-helical membrane proteins require additional restraints beyond the traditional Nuclear Overhauser Effect (NOE) restraints. Several methods have been developed and this review focuses on paramagnetic relaxation enhancement (PRE). Important aspects of spin labeling, PRE measurements, structure calculations, and structural quality are discussed.
© 2015 Elsevier Inc. All rights reserved.

Keywords:  Membrane proteins; Nitroxide spin label; Nuclear magnetic resonance; Paramagnetic relaxation enhancement; Polytopic; Site-directed spin labeling; α-Helical

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Year:  2015        PMID： 25950972     DOI： 10.1016/bs.mie.2014.12.005

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  2 in total

1.  Towards interpretation of intermolecular paramagnetic relaxation enhancement outside the fast exchange limit.

Authors:  Alberto Ceccon; G Marius Clore; Vitali Tugarinov
Journal:  J Biomol NMR       Date:  2016-08-24       Impact factor: 2.835

Review 2.  Applications of Solution NMR in Drug Discovery.

Authors:  Li Shi; Naixia Zhang
Journal:  Molecules       Date:  2021-01-22       Impact factor: 4.411
  2 in total

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