Literature DB >> 2594753

Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.

A Laban1, D F Wirth.   

Abstract

We report a transient expression transfection system in Leishmania enriettii. A hybrid gene containing an intergenic region of the alpha-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the alpha-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.

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Year:  1989        PMID： 2594753      PMCID： PMC298445          DOI： 10.1073/pnas.86.23.9119

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  35 in total

1.  A 5' spliced leader is added in trans to both alpha- and beta-tubulin transcripts in Trypanosoma brucei.

Authors:  S Sather; N Agabian
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

2.  Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis.

Authors:  M L Muhich; J C Boothroyd
Journal:  Mol Cell Biol       Date:  1988-09       Impact factor: 4.272

3.  Trans splicing involves a novel form of small nuclear ribonucleoprotein particles.

Authors:  J P Bruzik; K Van Doren; D Hirsh; J A Steitz
Journal:  Nature       Date:  1988-10-06       Impact factor: 49.962

4.  Tandem arrangement of tubulin genes in the protozoan parasite Leishmania enriettii.

Authors:  S M Landfear; D McMahon-Pratt; D F Wirth
Journal:  Mol Cell Biol       Date:  1983-06       Impact factor: 4.272

5.  The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's.

Authors:  J A Walder; P S Eder; D M Engman; S T Brentano; R Y Walder; D S Knutzon; D M Dorfman; J E Donelson
Journal:  Science       Date:  1986-08-01       Impact factor: 47.728

6.  Tubulin genes are tandemly linked and clustered in the genome of trypanosoma brucei.

Authors:  L S Thomashow; M Milhausen; W J Rutter; N Agabian
Journal:  Cell       Date:  1983-01       Impact factor: 41.582

7.  A 22-nucleotide spliced leader sequence in the human parasitic nematode Brugia malayi is identical to the trans-spliced leader exon in Caenorhabditis elegans.

Authors:  A M Takacs; J A Denker; K G Perrine; P A Maroney; T W Nilsen
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

8.  Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

Authors:  C M Gorman; L F Moffat; B H Howard
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Expression of a bacterial gene in a trypanosomatid protozoan.

Authors:  V Bellofatto; G A Cross
Journal:  Science       Date:  1989-06-09       Impact factor: 47.728
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  42 in total

1.  Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores.

Authors:  S I Sukharev; V A Klenchin; S M Serov; L V Chernomordik
Journal:  Biophys J       Date:  1992-11       Impact factor: 4.033

2.  Nuclease mapping and DNA sequence analysis of transcripts from the dihydrofolate reductase-thymidylate synthase (R) region of Leishmania major.

Authors:  G M Kapler; K Zhang; S M Beverley
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

3.  Pitfalls of the CAT reporter gene for analyzing translational regulation in Leishmania.

Authors:  Cristina Folgueira; Jose M Requena
Journal:  Parasitol Res       Date:  2007-08-05       Impact factor: 2.289

4.  Leishmania major Hsp100 is required chiefly in the mammalian stage of the parasite.

Authors:  A Hübel; S Krobitsch; A Hörauf; J Clos
Journal:  Mol Cell Biol       Date:  1997-10       Impact factor: 4.272

5.  Reduced antimony accumulation in ARM58-overexpressing Leishmania infantum.

Authors:  Carola Schäfer; Paloma Tejera Nevado; Dorothea Zander; Joachim Clos
Journal:  Antimicrob Agents Chemother       Date:  2013-12-23       Impact factor: 5.191

6.  A similar gene is shared by both the variant surface glycoprotein and procyclin gene transcription units of Trypanosoma brucei.

Authors:  M Berberof; A Pays; E Pays
Journal:  Mol Cell Biol       Date:  1991-03       Impact factor: 4.272

7.  Extrachromosomal genetic complementation of surface metalloproteinase (gp63)-deficient Leishmania increases their binding to macrophages.

Authors:  X Liu; K P Chang
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

8.  A Telomeric Cluster of Antimony Resistance Genes on Chromosome 34 of Leishmania infantum.

Authors:  Paloma Tejera Nevado; Eugenia Bifeld; Katharina Höhn; Joachim Clos
Journal:  Antimicrob Agents Chemother       Date:  2016-08-22       Impact factor: 5.191

9.  Transfection of the Giardia lamblia double-stranded RNA virus into giardia lamblia by electroporation of a single-stranded RNA copy of the viral genome.

Authors:  E S Furfine; C C Wang
Journal:  Mol Cell Biol       Date:  1990-07       Impact factor: 4.272

10.  The co-chaperone SGT of Leishmania donovani is essential for the parasite's viability.

Authors:  Gabi Ommen; Mareike Chrobak; Joachim Clos
Journal:  Cell Stress Chaperones       Date:  2009-12-02       Impact factor: 3.667
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