Hsi-Kung Kuo1,2, Yi-Hao Chen1,2, Faye Huang2,3, Yi-Chan Wu1, Jentaie Shiea4, Pei-Chang Wu5,6. 1. Department of Ophthalmology, Kaohsiung Chang-Gung Memorial Hospital, 123 Dapi Road, Niaosong District, Kaohsiung, 83301, Taiwan. 2. Chang-Gung University College of Medicine, Kaohsiung, Taiwan. 3. Department of Plastic and Reconstructive Surgery, Chang-Gung Memorial Hospital-Kaohsiung Medical Center, Kaohsiung, Taiwan. 4. Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan. 5. Department of Ophthalmology, Kaohsiung Chang-Gung Memorial Hospital, 123 Dapi Road, Niaosong District, Kaohsiung, 83301, Taiwan. wpc@adm.cgmh.org.tw. 6. Chang-Gung University College of Medicine, Kaohsiung, Taiwan. wpc@adm.cgmh.org.tw.
Abstract
PURPOSE: Proteins in the vitreous play an important role on the induction of proliferative vitreoretinopathy (PVR) after retinal detachment. The aim of this study was to investigate the variation of protein patterns in the vitreous of PVR eyes and examine whether differentially expressed protein levels were expressed in experimental PVR retina. METHODS: Vitreous samples from PVR and macular hole patients were selected for proteomic analysis. The vitreous protein samples were separated by two-dimensional electrophoresis (2-DE). The differentially expressed protein spots in the two groups were excised and subjected to in-gel digestion and identification by electrospray ionization mass spectrometry (ESI-MS) analysis. Two differentially expressed proteins, zinc finger protein 670 (ZFP 670) and prostaglandin D2 synthase (PGD2S), were further validated by immunohistochemical staining and western blotting analysis in the retina of the experimental rabbit PVR model. RESULTS: In proteome analysis of human vitreous samples, five proteins had increased expression in PVR, including zinc finger protein 670 (ZFP 670), prostaglandin D2 synthase (PGD2S), IgG (Immunoglobulin G) light chain, transthyretin precursor, and haptoglobin precursor. ZFP 670 and PGD2S levels were expressed significantly higher in the experimental PVR retinas than in the control group. CONCLUSIONS: Levels of ZFP 670 and PGD2S were elevated in the vitreous fluid of patients with PVR. In addition, there were higher expressions of ZFP 670 and PGD2S in the experimental PVR retina. This result will expand our knowledge of pathophysiologic characteristics of PVR, and might be helpful for further developing possible treatment on this disorder.
PURPOSE: Proteins in the vitreous play an important role on the induction of proliferative vitreoretinopathy (PVR) after retinal detachment. The aim of this study was to investigate the variation of protein patterns in the vitreous of PVR eyes and examine whether differentially expressed protein levels were expressed in experimental PVR retina. METHODS: Vitreous samples from PVR and macular hole patients were selected for proteomic analysis. The vitreous protein samples were separated by two-dimensional electrophoresis (2-DE). The differentially expressed protein spots in the two groups were excised and subjected to in-gel digestion and identification by electrospray ionization mass spectrometry (ESI-MS) analysis. Two differentially expressed proteins, zinc finger protein 670 (ZFP 670) and prostaglandin D2 synthase (PGD2S), were further validated by immunohistochemical staining and western blotting analysis in the retina of the experimental rabbit PVR model. RESULTS: In proteome analysis of human vitreous samples, five proteins had increased expression in PVR, including zinc finger protein 670 (ZFP 670), prostaglandin D2 synthase (PGD2S), IgG (Immunoglobulin G) light chain, transthyretin precursor, and haptoglobin precursor. ZFP 670 and PGD2S levels were expressed significantly higher in the experimental PVR retinas than in the control group. CONCLUSIONS: Levels of ZFP 670 and PGD2S were elevated in the vitreous fluid of patients with PVR. In addition, there were higher expressions of ZFP 670 and PGD2S in the experimental PVR retina. This result will expand our knowledge of pathophysiologic characteristics of PVR, and might be helpful for further developing possible treatment on this disorder.
Entities:
Keywords:
Electrospray ionization mass spectrometry; Proliferative vitreoretinopathy; Proteome; Two-dimensional electrophoresis; Zinc finger protein 670 and prostaglandin D2 synthase
Authors: Charanjit S Sethi; Geoffrey P Lewis; Steven K Fisher; William P Leitner; Derrick L Mann; Philip J Luthert; David G Charteris Journal: Invest Ophthalmol Vis Sci Date: 2005-01 Impact factor: 4.799