| Literature DB >> 25937170 |
Eyal Vardy1, J Elliott Robinson2, Chia Li3, Reid H J Olsen4, Jeffrey F DiBerto5, Patrick M Giguere1, Flori M Sassano1, Xi-Ping Huang1, Hu Zhu1, Daniel J Urban1, Kate L White1, Joseph E Rittiner6, Nicole A Crowley7, Kristen E Pleil7, Christopher M Mazzone7, Philip D Mosier8, Juan Song4, Thomas L Kash7, C J Malanga2, Michael J Krashes9, Bryan L Roth10.
Abstract
DREADDs are chemogenetic tools widely used to remotely control cellular signaling, neuronal activity, and behavior. Here we used a structure-based approach to develop a new Gi-coupled DREADD using the kappa-opioid receptor as a template (KORD) that is activated by the pharmacologically inert ligand salvinorin B (SALB). Activation of virally expressed KORD in several neuronal contexts robustly attenuated neuronal activity and modified behaviors. Additionally, co-expression of the KORD and the Gq-coupled M3-DREADD within the same neuronal population facilitated the sequential and bidirectional remote control of behavior. The availability of DREADDs activated by different ligands provides enhanced opportunities for investigating diverse physiological systems using multiplexed chemogenetic actuators.Entities:
Mesh：
Substances：
Year: 2015 PMID： 25937170 PMCID： PMC4441592 DOI： 10.1016/j.neuron.2015.03.065
Source DB: PubMed Journal: Neuron ISSN： 0896-6273 Impact factor: 17.173