Fabien Sauvet1, Catherine Drogou2, Clément Bougard2, Pierrick J Arnal3, Garance Dispersyn2, Cyprien Bourrilhon4, Arnaud Rabat2, Pascal Van Beers2, Danielle Gomez-Merino5, Brice Faraut6, Damien Leger6, Mounir Chennaoui2. 1. Institut de recherche biomédicale des armées(IRBA), Brétigny-sur-Orge, France; Université Paris Descartes, Hôtel Dieu, EA7330 VIFASOM (Vigilance, Fatigue, SOmmeil), Paris, France. Electronic address: fabien.sauvet@gmail.com. 2. Institut de recherche biomédicale des armées(IRBA), Brétigny-sur-Orge, France; Université Paris Descartes, Hôtel Dieu, EA7330 VIFASOM (Vigilance, Fatigue, SOmmeil), Paris, France. 3. Institut de recherche biomédicale des armées(IRBA), Brétigny-sur-Orge, France; Université Paris Descartes, Hôtel Dieu, EA7330 VIFASOM (Vigilance, Fatigue, SOmmeil), Paris, France; Université de Lyon, Laboratoire de Physiologie de l'Exercice, Saint Etienne, France. 4. Institut de recherche biomédicale des armées(IRBA), Brétigny-sur-Orge, France. 5. Université Paris Descartes, Hôtel Dieu, EA7330 VIFASOM (Vigilance, Fatigue, SOmmeil), Paris, France. 6. Université Paris Descartes, Hôtel Dieu, EA7330 VIFASOM (Vigilance, Fatigue, SOmmeil), Paris, France; Centre du Sommeil et de la Vigilance, Hôtel Dieu, APHP, Paris, France.
Abstract
BACKGROUND: Sleep loss may induce endothelial dysfunction, a key factor in cardiovascular risk. We examined the endothelial function during one week of sleep restriction and a recovery period (from 3-to-13 days) in healthy subjects, and its link to autonomic, inflammatory and/or endocrine responses. METHODS: 12 men were followed at baseline (B1, 8-h sleep), after 2 (SR2) and 6 (SR6) days of SR (4-h sleep: 02:00-06:00) and after 1 (R1) and 12 (R12) recovery nights (8h sleep). At 10:00, we assessed changes in: arm cutaneous vascular conductance (CVC) induced by local application of methacholine (MCh), cathodal current (CIV) and heat (44°C), finger CVC and skin temperature (Tfi) during local cold exposure (5°C, 20-min) and passive recovery (22°C, 20-min). Blood samples were collected at 08:00. RESULTS: Compared with baseline (B1), MCh and heat-induced maximal CVC values (CVC peak) were decreased at SR6 and R1. No effect of SR was observed for Tfi and CVC during immersion whereas these values were lower during passive recovery on SR6 and R1. From SR2 to R12, plasma concentrations of insulin, IGF-1 (total and free) and MCP-1 were significantly increased while those of testosterone and prolactin were decreased. Whole-blood blood mRNA concentrations of TNF-α and IL-1β were higher than B1. No changes in noradrenaline concentrations, heart rate and blood pressure were observed. CONCLUSIONS: These results demonstrate that SR reduces endothelial-dependent vasodilatation and local tolerance to cold. This endothelial dysfunction is independent of blood pressure and sympathetic activity but associated with inflammatory and metabolic pathway responses (ClinicalTrials-NCT01989741).
BACKGROUND: Sleep loss may induce endothelial dysfunction, a key factor in cardiovascular risk. We examined the endothelial function during one week of sleep restriction and a recovery period (from 3-to-13 days) in healthy subjects, and its link to autonomic, inflammatory and/or endocrine responses. METHODS: 12 men were followed at baseline (B1, 8-h sleep), after 2 (SR2) and 6 (SR6) days of SR (4-h sleep: 02:00-06:00) and after 1 (R1) and 12 (R12) recovery nights (8h sleep). At 10:00, we assessed changes in: arm cutaneous vascular conductance (CVC) induced by local application of methacholine (MCh), cathodal current (CIV) and heat (44°C), finger CVC and skin temperature (Tfi) during local cold exposure (5°C, 20-min) and passive recovery (22°C, 20-min). Blood samples were collected at 08:00. RESULTS: Compared with baseline (B1), MCh and heat-induced maximal CVC values (CVC peak) were decreased at SR6 and R1. No effect of SR was observed for Tfi and CVC during immersion whereas these values were lower during passive recovery on SR6 and R1. From SR2 to R12, plasma concentrations of insulin, IGF-1 (total and free) and MCP-1 were significantly increased while those of testosterone and prolactin were decreased. Whole-blood blood mRNA concentrations of TNF-α and IL-1β were higher than B1. No changes in noradrenaline concentrations, heart rate and blood pressure were observed. CONCLUSIONS: These results demonstrate that SR reduces endothelial-dependent vasodilatation and local tolerance to cold. This endothelial dysfunction is independent of blood pressure and sympathetic activity but associated with inflammatory and metabolic pathway responses (ClinicalTrials-NCT01989741).
Authors: Elissa K Hoopes; Felicia R Berube; Michele N D'Agata; Freda Patterson; William B Farquhar; David G Edwards; Melissa A H Witman Journal: Sleep Date: 2021-02-12 Impact factor: 5.849
Authors: Saurabh S Thosar; Alec M Berman; Maya X Herzig; Sally A Roberts; Michael R Lasarev; Steven A Shea Journal: Am J Physiol Regul Integr Comp Physiol Date: 2018-08-08 Impact factor: 3.619