| Literature DB >> 25931565 |
Markus Räschle1, Godelieve Smeenk2, Rebecca K Hansen2, Tikira Temu1, Yasuyoshi Oka2, Marco Y Hein1, Nagarjuna Nagaraj1, David T Long3, Johannes C Walter3, Kay Hofmann4, Zuzana Storchova5, Jürgen Cox1, Simon Bekker-Jensen6, Niels Mailand6, Matthias Mann7.
Abstract
DNA interstrand cross-links (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. We devised a technique called chromatin mass spectrometry (CHROMASS) to study protein recruitment dynamics during perturbed DNA replication in Xenopus egg extracts. Using CHROMASS, we systematically monitored protein assembly and disassembly on ICL-containing chromatin. Among numerous prospective DNA repair factors, we identified SLF1 and SLF2, which form a complex with RAD18 and together define a pathway that suppresses genome instability by recruiting the SMC5/6 cohesion complex to DNA lesions. Our study provides a global analysis of an entire DNA repair pathway and reveals the mechanism of SMC5/6 relocalization to damaged DNA in vertebrate cells.Entities:
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Year: 2015 PMID： 25931565 PMCID： PMC5331883 DOI： 10.1126/science.1253671
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728