Chaochao Tan1, Zongxin Ling, Ying Huang, Youde Cao, Qiang Liu, Tao Cai, Hao Yuan, Changjun Liu, Yunfeng Li, Keqian Xu. 1. From the *Department of Clinical Laboratory, Hunan Provincial People's Hospital; †Department of Clinical Biochemistry, Xiangya School of Medicine, Central South University, Changsha, Hunan; ‡Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang; §Department of Emergency, Hunan Provincial People's Hospital; ∥Department of Neurosurgery, the Third Xiangya Hospital, Central South University; and ¶Department of Gastroenterology, Hunan Provincial People's Hospital, Changsha, Hunan, China.
Abstract
OBJECTIVES: To evaluate alterations of the intestinal bacteria and its associations with the inflammation in acute pancreatitis (AP). METHODS: A multihospital prospective clinical study was conducted, and a total of 108 participants were enrolled in our study, including 44 with severe AP (SAP), 32 with mild AP (MAP), and 32 healthy volunteers. The structure of intestinal microbiota, 10 predominant bacteria, plasma endotoxin, and serum cytokines were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis, real-time quantitative polymerase chain reaction, Limulus amebocyte lysate tests, and enzyme-linked immunosorbent assays, respectively. RESULTS: Dramatic alterations in the predominant fecal microbiota were observed in most of both MAP and SAP patients. In addition, the rates of the multiorgan failures and infectious complications in the patients with SAP with altered intestinal microbiota were significantly higher than in those whose intestinal microbiota remained unaltered. Enterococcus increased and Bifidobacterium decreased in the patients with SAP compared to the patients with MAP. Serum IL-6 were positively correlated with Enterobacteriaceae and Enterococcus and negatively correlated with Bifidobacterium, whereas plasma endotoxin positively correlated with Enterococcus (P < 0.05). CONCLUSIONS: The intestinal bacteria most frequently altered in both the patients with MAP and those with SAP significantly correlated with inflammation, which indicated that the intestinal microbiota may be involved in the progression of AP.
OBJECTIVES: To evaluate alterations of the intestinal bacteria and its associations with the inflammation in acute pancreatitis (AP). METHODS: A multihospital prospective clinical study was conducted, and a total of 108 participants were enrolled in our study, including 44 with severe AP (SAP), 32 with mild AP (MAP), and 32 healthy volunteers. The structure of intestinal microbiota, 10 predominant bacteria, plasma endotoxin, and serum cytokines were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis, real-time quantitative polymerase chain reaction, Limulus amebocyte lysate tests, and enzyme-linked immunosorbent assays, respectively. RESULTS: Dramatic alterations in the predominant fecal microbiota were observed in most of both MAP and SAP patients. In addition, the rates of the multiorgan failures and infectious complications in the patients with SAP with altered intestinal microbiota were significantly higher than in those whose intestinal microbiota remained unaltered. Enterococcus increased and Bifidobacterium decreased in the patients with SAP compared to the patients with MAP. Serum IL-6 were positively correlated with Enterobacteriaceae and Enterococcus and negatively correlated with Bifidobacterium, whereas plasma endotoxin positively correlated with Enterococcus (P < 0.05). CONCLUSIONS: The intestinal bacteria most frequently altered in both the patients with MAP and those with SAP significantly correlated with inflammation, which indicated that the intestinal microbiota may be involved in the progression of AP.
Authors: Malini Ahuja; Daniella M Schwartz; Mayank Tandon; Aran Son; Mei Zeng; William Swaim; Michael Eckhaus; Victoria Hoffman; Yiyuan Cui; Bo Xiao; Paul F Worley; Shmuel Muallem Journal: Cell Metab Date: 2017-03-07 Impact factor: 27.287
Authors: L Brubaker; S Luu; Kl Hoffman; A Wood; M Navarro Cagigas; Q Yao; Jf Petrosino; W Fisher; G Van Buren Journal: Pancreatology Date: 2020-12-23 Impact factor: 3.996