Literature DB >> 25926484

Direct Detection of Influenza A and B Viruses in Less Than 20 Minutes Using a Commercially Available Rapid PCR Assay.

Matthew J Binnicker1, Mark J Espy2, Cole L Irish2, Emily A Vetter2.   

Abstract

We compared an FDA-cleared rapid (<20 min) PCR assay (Cobas Liat; Roche Diagnostics) to our routine influenza A and B real-time PCR assay (Simplexa Flu A/B & RSV Direct; Focus Diagnostics) using respiratory swabs (n = 197). The Cobas Liat influenza A and B assays demonstrated sensitivities of 99.2% (123/124) and 100% (23/23), respectively, while showing a specificity of 100% for each target.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.

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Year:  2015        PMID： 25926484      PMCID： PMC4473187          DOI： 10.1128/JCM.00791-15

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  6 in total

1.  Evaluation of the Cepheid Xpert Flu Assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses.

Authors:  S M Novak-Weekley; E M Marlowe; M Poulter; D Dwyer; D Speers; W Rawlinson; C Baleriola; C C Robinson
Journal:  J Clin Microbiol       Date:  2012-02-29       Impact factor: 5.948

2.  Pandemic H1N1 and seasonal H3N2 influenza infection in the human population show different distributions of viral loads, which substantially affect the performance of rapid influenza tests.

Authors:  Ji-Rong Yang; Je Lo; Yu-Lin Ho; Ho-Sheng Wu; Ming-Tsan Liu
Journal:  Virus Res       Date:  2010-09-25       Impact factor: 3.303

3.  Comparison of the Simplexa™ Flu A/B & RSV kit (nucleic acid extraction-dependent assay) and the Prodessa ProFlu+™ assay for detecting influenza and respiratory syncytial viruses.

Authors:  Suresh B Selvaraju; Adrienne V Bambach; Amy L Leber; Maria-Magdalena Patru; Anami Patel; Marilyn A Menegus
Journal:  Diagn Microbiol Infect Dis       Date:  2013-11-27       Impact factor: 2.803

4.  Comparison of real-time RT-PCR, shell vial culture, and conventional cell culture for the detection of the pandemic influenza A (H1N1) in hospitalized patients.

Authors:  Paula López Roa; Pilar Catalán; Maddalena Giannella; Darío García de Viedma; Virginia Sandonis; Emilio Bouza
Journal:  Diagn Microbiol Infect Dis       Date:  2011-04       Impact factor: 2.803

5.  Quantitative analysis of four rapid antigen assays for detection of pandemic H1N1 2009 compared with seasonal H1N1 and H3N2 influenza A viruses on nasopharyngeal aspirates from patients with influenza.

Authors:  K H Chan; K M Chan; Y L Ho; Y P Lam; H L Tong; L L M Poon; B J Cowling; J S M Peiris
Journal:  J Virol Methods       Date:  2012-09-07       Impact factor: 2.014

6.  Evaluation of 11 commercially available rapid influenza diagnostic tests--United States, 2011-2012.

Authors: 
Journal:  MMWR Morb Mortal Wkly Rep       Date:  2012-11-02       Impact factor: 17.586
  6 in total
  26 in total

1.  Assessment of Test Performance and Potential for Environmental Contamination Associated with a Point-of-Care Molecular Assay for Group A Streptococcus in an End User Setting.

Authors:  Leslie J Donato; Nikki K Myhre; Martha A Murray; Margaret R McDonah; Jane F Myers; Julie A Maxson; Alicia M Meek; Mark J Espy; Joseph W Furst; Brad S Karon; Matthew J Binnicker
Journal:  J Clin Microbiol       Date:  2019-01-30       Impact factor: 5.948

2.  Clinical Practice Guidelines by the Infectious Diseases Society of America: 2018 Update on Diagnosis, Treatment, Chemoprophylaxis, and Institutional Outbreak Management of Seasonal Influenzaa.

Authors:  Timothy M Uyeki; Henry H Bernstein; John S Bradley; Janet A Englund; Thomas M File; Alicia M Fry; Stefan Gravenstein; Frederick G Hayden; Scott A Harper; Jon Mark Hirshon; Michael G Ison; B Lynn Johnston; Shandra L Knight; Allison McGeer; Laura E Riley; Cameron R Wolfe; Paul E Alexander; Andrew T Pavia
Journal:  Clin Infect Dis       Date:  2019-03-05       Impact factor: 9.079

Review 3.  Point-of-Care Testing for Infectious Diseases: Past, Present, and Future.

Authors:  Thomas R Kozel; Amanda R Burnham-Marusich
Journal:  J Clin Microbiol       Date:  2017-05-24       Impact factor: 5.948

Review 4.  Rapid Antigen Tests for Influenza: Rationale and Significance of the FDA Reclassification.

Authors:  Daniel A Green; Kirsten StGeorge
Journal:  J Clin Microbiol       Date:  2018-09-25       Impact factor: 5.948

5.  Impact of Rapid Molecular Respiratory Virus Testing on Real-Time Decision Making in a Pediatric Emergency Department.

Authors:  Daniel T Rogan; Mohit S Kochar; Samuel Yang; James V Quinn
Journal:  J Mol Diagn       Date:  2017-03-22       Impact factor: 5.568

6.  Accurate PCR Detection of Influenza A/B and Respiratory Syncytial Viruses by Use of Cepheid Xpert Flu+RSV Xpress Assay in Point-of-Care Settings: Comparison to Prodesse ProFlu.

Authors:  Daniel M Cohen; Jennifer Kline; Larissa S May; Glenn Eric Harnett; Jane Gibson; Stephen Y Liang; Zubaid Rafique; Carina A Rodriguez; Kevin M McGann; Charlotte A Gaydos; Donna Mayne; David Phillips; Jason Cohen
Journal:  J Clin Microbiol       Date:  2018-01-24       Impact factor: 5.948

7.  Direct Comparison of Alere i and cobas Liat Influenza A and B Tests for Rapid Detection of Influenza Virus Infection.

Authors:  Frederick S Nolte; Lori Gauld; Susan B Barrett
Journal:  J Clin Microbiol       Date:  2016-08-31       Impact factor: 5.948

Review 8.  New Developments in Clinical Bacteriology Laboratories.

Authors:  Robin Patel
Journal:  Mayo Clin Proc       Date:  2016-08-21       Impact factor: 7.616

9.  Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections.

Authors:  Carmen L Charlton; Esther Babady; Christine C Ginocchio; Todd F Hatchette; Robert C Jerris; Yan Li; Mike Loeffelholz; Yvette S McCarter; Melissa B Miller; Susan Novak-Weekley; Audrey N Schuetz; Yi-Wei Tang; Ray Widen; Steven J Drews
Journal:  Clin Microbiol Rev       Date:  2018-12-12       Impact factor: 26.132

10.  Clinical Impact of Rapid Point-of-Care PCR Influenza Testing in an Urgent Care Setting: a Single-Center Study.

Authors:  Robert C Benirschke; Erin McElvania; Richard B Thomson; Karen L Kaul; Sanchita Das
Journal:  J Clin Microbiol       Date:  2019-02-27       Impact factor: 5.948
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