| Literature DB >> 25921091 |
Juan R Alvarez-Dominguez1,2, Zhiqiang Bai3, Dan Xu3, Bingbing Yuan1, Kinyui Alice Lo4, Myeong Jin Yoon3, Yen Ching Lim3, Marko Knoll1, Nikolai Slavov2, Shuai Chen5, Chen Peng6, Harvey F Lodish1,2, Lei Sun3,4.
Abstract
Brown adipose tissue (BAT) protects against obesity by promoting energy expenditure via uncoupled respiration. To uncover BAT-specific long non-coding RNAs (lncRNAs), we used RNA-seq to reconstruct de novo transcriptomes of mouse brown, inguinal white, and epididymal white fat and identified ∼1,500 lncRNAs, including 127 BAT-restricted loci induced during differentiation and often targeted by key regulators PPARγ, C/EBPα, and C/EBPβ. One of them, lnc-BATE1, is required for establishment and maintenance of BAT identity and thermogenic capacity. lnc-BATE1 inhibition impairs concurrent activation of brown fat and repression of white fat genes and is partially rescued by exogenous lnc-BATE1 with mutated siRNA-targeting sites, demonstrating a function in trans. We show that lnc-BATE1 binds heterogeneous nuclear ribonucleoprotein U and that both are required for brown adipogenesis. Our work provides an annotated catalog for the study of fat depot-selective lncRNAs and establishes lnc-BATE1 as a regulator of BAT development and physiology.Entities:
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Year: 2015 PMID： 25921091 PMCID： PMC4429916 DOI： 10.1016/j.cmet.2015.04.003
Source DB: PubMed Journal: Cell Metab ISSN： 1550-4131 Impact factor: 27.287