| Literature DB >> 25916709 |
Shaodong Jia1, Lisette Marjavaara, Robert Buckland, Sushma Sharma, Andrei Chabes.
Abstract
DNA polymerase assays are commonly used for the detection of deoxyribonucleoside triphosphates (dNTPs) in biological samples. For better specificity and accuracy, high-performance liquid chromatography (HPLC) methods have been developed for the analysis of the four dNTPs in complex samples. Here we describe a simple method using isocratic strong anion-exchange (SAX) chromatographic separation coupled with ultraviolet detection (UV) for the analysis of the four dNTPs in budding yeast Saccharomyces cerevisiae. This method can be applied to other species of yeast or bacteria.Entities:
Mesh：
Substances：
Year: 2015 PMID： 25916709 DOI： 10.1007/978-1-4939-2596-4_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745