Literature DB >> 25908561

Conditions for (13)C NMR detection of 2-hydroxyglutarate in tissue extracts from isocitrate dehydrogenase-mutated gliomas.

Kumar Pichumani1, Tomoyuki Mashimo2, Hyeon-Man Baek3, James Ratnakar4, Bruce Mickey5, Ralph J DeBerardinis6, Elizabeth A Maher7, Robert M Bachoo7, Craig R Malloy8, Zoltan Kovacs4.   

Abstract

(13)C NMR (nuclear magnetic resonance) spectroscopy of extracts from patient tumor samples provides rich information about metabolism. However, in isocitrate dehydrogenase (IDH)-mutant gliomas, (13)C labeling is obscured in oncometabolite 2-hydroxyglutaric acid (2 HG) by glutamate and glutamine, prompting development of a simple method to resolve the metabolites. J-coupled multiplets in 2 HG were similar to glutamate and glutamine and could be clearly resolved at pH 6. A cryogenically cooled (13)C probe, but not J-resolved heteronuclear single quantum coherence spectroscopy, significantly improved detection of 2 HG. These methods enable the monitoring of (13)C-(13)C spin-spin couplings in 2 HG expressing IDH-mutant gliomas.
Copyright © 2015 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  (13)C NMR; 2-Hydroxyglutarate; Cryoprobe; IDH-mutant gliomas

Mesh：

Substances：

Year:  2015        PMID： 25908561      PMCID： PMC4458399          DOI： 10.1016/j.ab.2015.04.017

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  15 in total

1.  13C isotopomer analysis of glutamate by J-resolved heteronuclear single quantum coherence spectroscopy.

Authors:  S C Burgess; R A Carvalho; M E Merritt; J G Jones; C R Malloy; A D Sherry
Journal:  Anal Biochem       Date:  2001-02-15       Impact factor: 3.365

2.  Non-invasive in vivo assessment of IDH1 mutational status in glioma.

Authors:  Myriam M Chaumeil; Peder E Z Larson; Hikari A I Yoshihara; Olivia M Danforth; Daniel B Vigneron; Sarah J Nelson; Russell O Pieper; Joanna J Phillips; Sabrina M Ronen
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3.  Carbon-13 nuclear magnetic resonance titration shifts in amino acids.

Authors:  A R Quirt; J R Lyerla; I R Peat; J S Cohen; W F Reynolds; M H Freedman
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4.  Acetate is a bioenergetic substrate for human glioblastoma and brain metastases.

Authors:  Tomoyuki Mashimo; Kumar Pichumani; Vamsidhara Vemireddy; Kimmo J Hatanpaa; Dinesh Kumar Singh; Shyam Sirasanagandla; Suraj Nannepaga; Sara G Piccirillo; Zoltan Kovacs; Chan Foong; Zhiguang Huang; Samuel Barnett; Bruce E Mickey; Ralph J DeBerardinis; Benjamin P Tu; Elizabeth A Maher; Robert M Bachoo
Journal:  Cell       Date:  2014-12-18       Impact factor: 41.582

5.  Detection of 2-hydroxyglutarate in IDH-mutated glioma patients by in vivo spectral-editing and 2D correlation magnetic resonance spectroscopy.

Authors:  Ovidiu C Andronesi; Grace S Kim; Elizabeth Gerstner; Tracy Batchelor; Aria A Tzika; Valeria R Fantin; Matthew G Vander Heiden; A Gregory Sorensen
Journal:  Sci Transl Med       Date:  2012-01-11       Impact factor: 17.956

6.  Metabolism of [U-13 C]glucose in human brain tumors in vivo.

Authors:  Elizabeth A Maher; Isaac Marin-Valencia; Robert M Bachoo; Tomoyuki Mashimo; Jack Raisanen; Kimmo J Hatanpaa; Ashish Jindal; F Mark Jeffrey; Changho Choi; Christopher Madden; Dana Mathews; Juan M Pascual; Bruce E Mickey; Craig R Malloy; Ralph J DeBerardinis
Journal:  NMR Biomed       Date:  2012-03-15       Impact factor: 4.044

7.  Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.

Authors:  Lenny Dang; David W White; Stefan Gross; Bryson D Bennett; Mark A Bittinger; Edward M Driggers; Valeria R Fantin; Hyun Gyung Jang; Shengfang Jin; Marie C Keenan; Kevin M Marks; Robert M Prins; Patrick S Ward; Katharine E Yen; Linda M Liau; Joshua D Rabinowitz; Lewis C Cantley; Craig B Thompson; Matthew G Vander Heiden; Shinsan M Su
Journal:  Nature       Date:  2009-12-10       Impact factor: 49.962

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Journal:  Science       Date:  2013-04-04       Impact factor: 47.728

9.  2-hydroxyglutarate detection by magnetic resonance spectroscopy in IDH-mutated patients with gliomas.

Authors:  Changho Choi; Sandeep K Ganji; Ralph J DeBerardinis; Kimmo J Hatanpaa; Dinesh Rakheja; Zoltan Kovacs; Xiao-Li Yang; Tomoyuki Mashimo; Jack M Raisanen; Isaac Marin-Valencia; Juan M Pascual; Christopher J Madden; Bruce E Mickey; Craig R Malloy; Robert M Bachoo; Elizabeth A Maher
Journal:  Nat Med       Date:  2012-01-26       Impact factor: 53.440

10.  Detection of "oncometabolite" 2-hydroxyglutarate by magnetic resonance analysis as a biomarker of IDH1/2 mutations in glioma.

Authors:  Hui Mao; Erwin G Van Meir; Juliya Kalinina; Anne Carroll; Liya Wang; Qiqi Yu; Danny E Mancheno; Shaoxiong Wu; Frank Liu; Jun Ahn; Miao He
Journal:  J Mol Med (Berl)       Date:  2012-03-17       Impact factor: 5.606
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  6 in total

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Journal:  Mol Carcinog       Date:  2017-07-17       Impact factor: 4.784

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Authors:  Victor Ruiz-Rodado; Adrian Lita; Tyrone Dowdy; Orieta Celiku; Alejandra Cavazos Saldana; Herui Wang; Chun Zhang Yang; Raj Chari; Aiguo Li; Wei Zhang; Hua Song; Meili Zhang; Susie Ahn; Dionne Davis; Xiang Chen; Zhengping Zhuang; Christel Herold-Mende; Kylie J Walters; Mark R Gilbert; Mioara Larion
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3.  Lactate Dehydrogenase C Produces S-2-Hydroxyglutarate in Mouse Testis.

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Review 4.  Molecular Imaging of Metabolic Reprograming in Mutant IDH Cells.

Authors:  Pavithra Viswanath; Myriam M Chaumeil; Sabrina M Ronen
Journal:  Front Oncol       Date:  2016-03-14       Impact factor: 6.244

5.  Quantitative Imaging of D-2-Hydroxyglutarate in Selected Histological Tissue Areas by a Novel Bioluminescence Technique.

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Journal:  Front Oncol       Date:  2016-03-07       Impact factor: 6.244

6.  Magnetic resonance spectroscopy for the study of cns malignancies.

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  6 in total

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