Literature DB >> 11161312

13C isotopomer analysis of glutamate by J-resolved heteronuclear single quantum coherence spectroscopy.

S C Burgess1, R A Carvalho, M E Merritt, J G Jones, C R Malloy, A D Sherry.   

Abstract

13C NMR isotopomer analysis is a powerful method for measuring metabolic fluxes through pathways intersecting in the tricarboxylic acid cycle. However, the inherent insensitivity of 13C NMR spectroscopy makes application of isotopomer analysis to small tissue samples (mouse tissue, human biopsies, or cells grown in tissue culture) problematic. (1)H NMR is intrinsically more sensitive than 13C NMR and can potentially supply the same information via indirect detection of 13C providing that isotopomer information can be preserved. We report here the use of J-resolved HSQC (J-HSQC) for 13C isotopomer analysis of tissue samples. We show that J-HSQC reports isotopomer multiplet patterns identical to those reported by direct 13C detection but with improved sensitivity. Copyright 2001 Academic Press.

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Year:  2001        PMID： 11161312     DOI： 10.1006/abio.2000.4930

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  Conditions for (13)C NMR detection of 2-hydroxyglutarate in tissue extracts from isocitrate dehydrogenase-mutated gliomas.

Authors:  Kumar Pichumani; Tomoyuki Mashimo; Hyeon-Man Baek; James Ratnakar; Bruce Mickey; Ralph J DeBerardinis; Elizabeth A Maher; Robert M Bachoo; Craig R Malloy; Zoltan Kovacs
Journal:  Anal Biochem       Date:  2015-04-20       Impact factor: 3.365

2.  Multiple mass isotopomer tracing of acetyl-CoA metabolism in Langendorff-perfused rat hearts: channeling of acetyl-CoA from pyruvate dehydrogenase to carnitine acetyltransferase.

Authors:  Qingling Li; Shuang Deng; Rafael A Ibarra; Vernon E Anderson; Henri Brunengraber; Guo-Fang Zhang
Journal:  J Biol Chem       Date:  2015-02-02       Impact factor: 5.157

Review 3.  Assessing Cardiac Metabolism: A Scientific Statement From the American Heart Association.

Authors:  Heinrich Taegtmeyer; Martin E Young; Gary D Lopaschuk; E Dale Abel; Henri Brunengraber; Victor Darley-Usmar; Christine Des Rosiers; Robert Gerszten; Jan F Glatz; Julian L Griffin; Robert J Gropler; Hermann-Georg Holzhuetter; Jorge R Kizer; E Douglas Lewandowski; Craig R Malloy; Stefan Neubauer; Linda R Peterson; Michael A Portman; Fabio A Recchia; Jennifer E Van Eyk; Thomas J Wang
Journal:  Circ Res       Date:  2016-03-24       Impact factor: 17.367

4.  Effects of deuteration on transamination and oxidation of hyperpolarized 13C-Pyruvate in the isolated heart.

Authors:  Alexander M Funk; Xiaodong Wen; Thomas Hever; Nesmine R Maptue; Chalermchai Khemtong; A D Sherry; Craig R Malloy
Journal:  J Magn Reson       Date:  2019-03-04       Impact factor: 2.229

5.  Using 31P NMR spectroscopy at 14.1 Tesla to investigate PARP-1 associated energy failure and metabolic rescue in cerebrocortical slices.

Authors:  Jianying Zeng; Kiyoshi Hirai; Guo-Yuan Yang; Weihai Ying; Raymond A Swanson; Mark Kelly; Moriz Mayer; Thomas L James; Lawrence Litt
Journal:  J Bioenerg Biomembr       Date:  2004-08       Impact factor: 2.945

6.  Resolving the sources of plasma glucose excursions following a glucose tolerance test in the rat with deuterated water and [U-13C]glucose.

Authors:  Teresa C Delgado; Cristina Barosa; Patrícia M Nunes; Sebastián Cerdán; Carlos F G C Geraldes; John G Jones
Journal:  PLoS One       Date:  2012-03-30       Impact factor: 3.240
  6 in total

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