| Literature DB >> 25908559 |
Smitha Sivapragasam1, Anuja Pande1, Anne Grove2.
Abstract
Fragment analysis was developed to determine the sizes of DNA fragments relative to size standards of known lengths using a capillary electrophoresis genetic analyzer. This approach has since been adapted for use in DNA footprinting. However, DNA footprinting requires accurate determination of both fragment length and intensity, imposing specific demands on the experimental design. Here we delineate essential considerations involved in optimizing the fragment analysis workflow for use in DNase I footprinting to ensure that changes in DNase I cleavage patterns may be reliably identified.Keywords: Capillary electrophoresis; DNase I footprinting; Fragment analysis; Single hit
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Year: 2015 PMID： 25908559 DOI： 10.1016/j.ab.2015.04.013
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365