BACKGROUND: Gastric cancer results from a multifactorial process and is one of the most common causes of death worldwide. These tumors can arise in the distal stomach (non-cardia) and in the cardia region, presenting different characteristics and frequency of occurrence worldwide. AIMS: To search for differences between tumors of different locations that could explain the presence of cardia tumors, considering Helicobacter pylori strains and genetic polymorphisms. MATERIALS AND METHODS: DNA was extracted from gastric adenocarcinoma tissue of 127 patients. Helicobacter pylori genes were detected by PCR, and polymorphisms by PCR-RFLP. RESULTS: Most of the tumors were located in non-cardia. The genotype 28152GA of XRCC1 showed an increase in risk of cardia tumors. In analysis performed considering gender, women carrying TNF-308GA genotype showed a decreased risk of non-cardia tumors, while in men the decreased risk of non-cardia tumors was associated with TNF-308GG genotype. Genotypes combinations showed that the SNPs RAD51 135G>C, XRCC3 18067C>T, and XRCC1 28152G>A had some combinations more frequent in cardia tumors, with an increased risk. Patients infected by cagE-positive strains presented a positive correlation with non-cardia tumors. CONCLUSION: The results showed some susceptibility differences between tumors of different locations. There was an increased risk relationship between three repair enzyme SNPs and cardia tumors, and the G allele of the cytokine gene TNF negatively influenced the development of non-cardia tumors. Helicobacter pylori strains seemed to be different in the cardia region, where they were less virulent than those located in the distal region of the stomach.
BACKGROUND: Gastric cancer results from a multifactorial process and is one of the most common causes of death worldwide. These tumors can arise in the distal stomach (non-cardia) and in the cardia region, presenting different characteristics and frequency of occurrence worldwide. AIMS: To search for differences between tumors of different locations that could explain the presence of cardia tumors, considering Helicobacter pylori strains and genetic polymorphisms. MATERIALS AND METHODS: DNA was extracted from gastric adenocarcinoma tissue of 127 patients. Helicobacter pylori genes were detected by PCR, and polymorphisms by PCR-RFLP. RESULTS: Most of the tumors were located in non-cardia. The genotype 28152GA of XRCC1 showed an increase in risk of cardia tumors. In analysis performed considering gender, women carrying TNF-308GA genotype showed a decreased risk of non-cardia tumors, while in men the decreased risk of non-cardia tumors was associated with TNF-308GG genotype. Genotypes combinations showed that the SNPs RAD51 135G>C, XRCC3 18067C>T, and XRCC1 28152G>A had some combinations more frequent in cardia tumors, with an increased risk. Patients infected by cagE-positive strains presented a positive correlation with non-cardia tumors. CONCLUSION: The results showed some susceptibility differences between tumors of different locations. There was an increased risk relationship between three repair enzyme SNPs and cardia tumors, and the G allele of the cytokine gene TNF negatively influenced the development of non-cardia tumors. Helicobacter pylori strains seemed to be different in the cardia region, where they were less virulent than those located in the distal region of the stomach.
Authors: Svein Hansen; Stein Emil Vollset; Mohammad H Derakhshan; Valerie Fyfe; Kjetil K Melby; Steinar Aase; Egil Jellum; Kenneth E L McColl Journal: Gut Date: 2007-02-22 Impact factor: 23.059
Authors: Valeska Portela Lima; Isabelle Joyce de Lima Silva-Fernandes; Markênia Kélia Santos Alves; Silvia Helena Barem Rabenhorst Journal: Cancer Epidemiol Date: 2011-04-05 Impact factor: 2.984
Authors: Patricia Carrera-Lasfuentes; Angel Lanas; Luis Bujanda; Mark Strunk; Enrique Quintero; Santos Santolaria; Rafael Benito; Federico Sopeña; Elena Piazuelo; Concha Thomson; Angeles Pérez-Aisa; David Nicolás-Pérez; Elizabeth Hijona; Jesús Espinel; Rafael Campo; Marisa Manzano; Fernando Geijo; María Pellise; Manuel Zaballa; Ferrán González-Huix; Jorge Espinós; Llúcia Titó; Luis Barranco; Mauro D'Amato; María Asunción García-González Journal: Oncotarget Date: 2017-05-30