Thiago Henrique Scarabello Stape1, Leo Tjäderhane2, Marcelo Rocha Marques3, Flávio Henrique Baggio Aguiar4, Luís Roberto Marcondes Martins5. 1. Piracicaba Dental School, University of Campinas - UNICAMP, Department of Restorative Dentistry, Av. Limeira 901, Piracicaba, SP 13414-903, Brazil. Electronic address: thiagohs@hotmail.com. 2. Institute of Dentistry, University of Oulu, and Medical Research Center Oulu, Oulu University Hospital, PO Box 5281, 90014 University of Oulu, Finland; Department of Oral and Maxillofacial Diseases, University of Helsinki, Helsinki University Hospital, Helsinki, Finland. Electronic address: leo.tjaderhane@oulu.fi. 3. Piracicaba Dental School, University of Campinas - UNICAMP, Department of Morphology, Av. Limeira 901, Piracicaba, SP 13414-903, Brazil. Electronic address: marques_fop@hotmail.com. 4. Piracicaba Dental School, University of Campinas - UNICAMP, Department of Restorative Dentistry, SP 13414-903, Brazil. Electronic address: aguiar@fop.unicamp.br. 5. Piracicaba Dental School, University of Campinas - UNICAMP, Department of Restorative Dentistry, SP 13414-903, Brazil. Electronic address: martins@fop.unicamp.br.
Abstract
OBJECTIVES: This study examined the effect of a dimethyl sulfoxide (DMSO) wet bonding technique on the resin infiltration depths at the bonded interface and dentin bond strength of different adhesive systems. METHODS: Flat dentin surfaces of 48 human third molars were treated with 50% DMSO (experimental groups) or with distilled water (controls) before bonding using an etch-and-rinse (SBMP: Scotchbond Multi-Purpose, 3M ESPE) or a self-etch (Clearfil: Clearfil SE Bond, Kuraray) adhesive system. The restored crown segments (n=12/group) were stored in distilled water (24h) and sectioned for interfacial analysis of exposed collagen using Masson's Trichrome staining and for microtensile bond strength testing. The extent of exposed collagen was measured using light microscopy and a histometric analysis software. Failure modes were examined by SEM. Data was analyzed by two-way ANOVA followed by Tukey Test (α=0.05). RESULTS: The interaction of bonding protocol and adhesive system had significant effects on the extension of exposed collagen matrix (p<0.0001) and bond strength (p=0.0091). DMSO-wet bonding significantly reduced the extent of exposed collagen matrix for SBMP and Clearfil (p<0.05). Significant increase in dentin bond strength was observed on DMSO-treated specimens bonded with SBMP (p<0.05), while no differences were observed for Clearfil (p>0.05). SIGNIFICANCE: DMSO-wet bonding was effective to improve the quality of resin-dentin bonds of the tested etch-and-rinse adhesives by reducing the extent of exposed collagen matrix at the base of the resin-dentin biopolymer. The improved penetration of adhesive monomers is reflected as an increase in the immediate bond strength when the DMSO-wet bonding technique is used with a water-based etch-and-rinse adhesive.
OBJECTIVES: This study examined the effect of a dimethyl sulfoxide (DMSO) wet bonding technique on the resin infiltration depths at the bonded interface and dentin bond strength of different adhesive systems. METHODS: Flat dentin surfaces of 48 human third molars were treated with 50% DMSO (experimental groups) or with distilled water (controls) before bonding using an etch-and-rinse (SBMP: Scotchbond Multi-Purpose, 3M ESPE) or a self-etch (Clearfil: Clearfil SE Bond, Kuraray) adhesive system. The restored crown segments (n=12/group) were stored in distilled water (24h) and sectioned for interfacial analysis of exposed collagen using Masson's Trichrome staining and for microtensile bond strength testing. The extent of exposed collagen was measured using light microscopy and a histometric analysis software. Failure modes were examined by SEM. Data was analyzed by two-way ANOVA followed by Tukey Test (α=0.05). RESULTS: The interaction of bonding protocol and adhesive system had significant effects on the extension of exposed collagen matrix (p<0.0001) and bond strength (p=0.0091). DMSO-wet bonding significantly reduced the extent of exposed collagen matrix for SBMP and Clearfil (p<0.05). Significant increase in dentin bond strength was observed on DMSO-treated specimens bonded with SBMP (p<0.05), while no differences were observed for Clearfil (p>0.05). SIGNIFICANCE: DMSO-wet bonding was effective to improve the quality of resin-dentin bonds of the tested etch-and-rinse adhesives by reducing the extent of exposed collagen matrix at the base of the resin-dentin biopolymer. The improved penetration of adhesive monomers is reflected as an increase in the immediate bond strength when the DMSO-wet bonding technique is used with a water-based etch-and-rinse adhesive.
Authors: Magali Inglês; Joana Vasconcelos E Cruz; Ana Mano Azul; Mário Polido; António H S Delgado Journal: Polymers (Basel) Date: 2022-09-21 Impact factor: 4.967