| Literature DB >> 25896454 |
Li Wang1, Hong Li1, Xueqin Lv2, Tong Chen3, Ruili Li4, Yiqun Xue3, Jianjun Jiang5, Biao Jin6, František Baluška7, Jozef Šamaj8, Xuelu Wang9, Jinxing Lin10.
Abstract
The major brassinosteroid (BR) receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 (BRI1) plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization and assembly state remain unclear. Here, we applied variable angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy to analyze the dynamics of GFP-tagged BRI1. We found that, in response to BR, the degree of co-localization of BRI1-GFP with AtFlot1-mCherry increased, and especially BR stimulated the membrane microdomain-associated pathway of BRI1 internalization. We also verified these observations in endocytosis-defective chc2-1 mutants and the AtFlot1 amiRNA 15-5 lines. Furthermore, examination of the phosphorylation status of bri1-EMS-suppressor 1 and measurement of BR-responsive gene expression revealed that membrane microdomains affect BR signaling. These results suggest that BR promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling.Entities:
Keywords: BR signaling; BRI1; endocytosis; membrane microdomains; spatiotemporal dynamics
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Year: 2015 PMID： 25896454 DOI： 10.1016/j.molp.2015.04.005
Source DB: PubMed Journal: Mol Plant ISSN： 1674-2052 Impact factor: 13.164