| Literature DB >> 31431511 |
Xi Zhang1,2, Yaning Cui1,2, Meng Yu1,2, Bodan Su1,2, Wei Gong1,2, František Baluška3, George Komis4, Jozef Šamaj4, Xiaoyi Shan5,2, Jinxing Lin1,2.
Abstract
The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and signaling, governed by Thr-101 (T101) phosphorylation. NRT1.1 regulates lateral root (LR) development by modulating nitrate-dependent basipetal auxin export and nitrate-mediated signal transduction. Here, using the Arabidopsis (Arabidopsis thaliana) NRT1.1T101D phosphomimetic and NRT1.1T101A nonphosphorylatable mutants, we found that the phosphorylation state of NRT1.1 plays a key role in NRT1.1 function during LR development. Single-particle tracking revealed that phosphorylation affected NRT1.1 spatiotemporal dynamics. The phosphomimetic NRT1.1T101D form showed fast lateral mobility and membrane partitioning that facilitated auxin flux under low-nitrate conditions. By contrast, nonphosphorylatable NRT1.1T101A showed low lateral mobility and oligomerized at the plasma membrane (PM), where it induced endocytosis via the clathrin-mediated endocytosis and microdomain-mediated endocytosis pathways under high-nitrate conditions. These behaviors promoted LR development by suppressing NRT1.1-controlled auxin transport on the PM and stimulating Ca2+-ARABIDOPSIS NITRATE REGULATED1 signaling from the endosome.Entities:
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Year: 2019 PMID： 31431511 PMCID： PMC6776865 DOI： 10.1104/pp.19.00346
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340