Nocardioides solisilvae sp. nov., isolated from a forest soil.

A Gram-stain positive, rod shaped aerobic bacterial strain designated Ka25(T) was isolated from a forest soil sample near Kasol, Himachal Pradesh, India. Based on 16S rRNA gene sequence analysis it was identified that strain Ka25(T) belongs to the class Actinobacteria and is most closely related to Nocardioides daejeonensis KCTC 19772(T) (97.2 %), Nocardioides dubius JCM 13008(T) (96.9 %), Nocardioides psychrotolerans CGMCC 1.11156(T) (96.8 %), Nocardioides pacificus DSM 27278(T)(96.7 %) and Nocardioides daphniae JCM 16608(T)(96.4 %) sequence similarity respectively. The chemotaxonomic properties of strain Ka25(T) are consistent with those of the members of the genus Nocardioides: MK-8(H4) is the predominant menaquinone, iso-C16 : 0, iso-C17 : 0 and C18 : 1ω9c were identified as the predominant cellular fatty acids and the cell-wall peptidoglycan is based on LL-2,6-diaminopimelic acid. The DNA G+C content of strain Ka25(T) was determined to be 71.6 mol %. The results of the polyphasic taxonomic analysis allowed a clear differentiation of strain Ka25(T) from all other members of the genus Nocardioides. Strain Ka25(T) is thus considered to represent a novel member of the genus Nocardioides, for which the name Nocardioides solisilvae sp. nov. is proposed. The type strain is Ka25(T) (=KCTC39528(T) =MCC2762(T)).