| Literature DB >> 25889806 |
Chiara Samorì1, Federica Abbondanzi2, Paola Galletti3, Loris Giorgini4, Laura Mazzocchetti5, Cristian Torri3, Emilio Tagliavini3.
Abstract
Polyhydroxyalkanoates (PHAs) can be extracted from mixed microbial cultures (MMCs) by means of dimethyl carbonate (DMC) or combination of DMC and sodium hypochlorite (NaClO). The protocol based on DMC, a green solvent never used before for the extraction of PHAs from MMC, allows an overall polymer recovery of 63%; also the purity and the molecular weight of the recovered polymers are good (98% and 1.2 MDa, respectively). The use of NaClO pretreatment before DMC extraction increases the overall PHA recovery (82%) but lowers the mean molecular weight to 0.6-0.2 MDa. A double extraction with DMC results to be the method of choice for the recovery of high quality PHAs from attractive but challenging MMCs.Entities:
Keywords: Dimethyl carbonate; Mixed microbial cultures; Polyhydroxyalkanoates; Polymer extraction
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Year: 2015 PMID: 25889806 DOI: 10.1016/j.biortech.2015.03.062
Source DB: PubMed Journal: Bioresour Technol ISSN: 0960-8524 Impact factor: 9.642