Literature DB >> 25889806

Extraction of polyhydroxyalkanoates from mixed microbial cultures: Impact on polymer quality and recovery.

Chiara Samorì1, Federica Abbondanzi2, Paola Galletti3, Loris Giorgini4, Laura Mazzocchetti5, Cristian Torri3, Emilio Tagliavini3.   

Abstract

Polyhydroxyalkanoates (PHAs) can be extracted from mixed microbial cultures (MMCs) by means of dimethyl carbonate (DMC) or combination of DMC and sodium hypochlorite (NaClO). The protocol based on DMC, a green solvent never used before for the extraction of PHAs from MMC, allows an overall polymer recovery of 63%; also the purity and the molecular weight of the recovered polymers are good (98% and 1.2 MDa, respectively). The use of NaClO pretreatment before DMC extraction increases the overall PHA recovery (82%) but lowers the mean molecular weight to 0.6-0.2 MDa. A double extraction with DMC results to be the method of choice for the recovery of high quality PHAs from attractive but challenging MMCs.
Copyright © 2015 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Dimethyl carbonate; Mixed microbial cultures; Polyhydroxyalkanoates; Polymer extraction

Mesh:

Substances:

Year:  2015        PMID: 25889806     DOI: 10.1016/j.biortech.2015.03.062

Source DB:  PubMed          Journal:  Bioresour Technol        ISSN: 0960-8524            Impact factor:   9.642


  8 in total

1.  Quantitative image analysis of polyhydroxyalkanoates inclusions from microbial mixed cultures under different SBR operation strategies.

Authors:  António L Amaral; Hugo Abreu; Cristiano Leal; Daniela P Mesquita; Luís M Castro; Eugénio C Ferreira
Journal:  Environ Sci Pollut Res Int       Date:  2017-05-12       Impact factor: 4.223

2.  Polyhydroxyalkanoates from a Mixed Microbial Culture: Extraction Optimization and Polymer Characterization.

Authors:  Ana Marta Rodrigues; Rita Dias Guardão Franca; Madalena Dionísio; Chantal Sevrin; Christian Grandfils; Maria A M Reis; Nídia Dana Lourenço
Journal:  Polymers (Basel)       Date:  2022-05-25       Impact factor: 4.967

Review 3.  Biological Approaches in Polyhydroxyalkanoates Recovery.

Authors:  K Gonzalez; R Navia; Shijie Liu; Mara Cea
Journal:  Curr Microbiol       Date:  2020-10-28       Impact factor: 2.188

Review 4.  Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production.

Authors:  Constantina Kourmentza; Jersson Plácido; Nikolaos Venetsaneas; Anna Burniol-Figols; Cristiano Varrone; Hariklia N Gavala; Maria A M Reis
Journal:  Bioengineering (Basel)       Date:  2017-06-11

Review 5.  Recovery of Polyhydroxyalkanoates From Single and Mixed Microbial Cultures: A Review.

Authors:  Giorgia Pagliano; Paola Galletti; Chiara Samorì; Agnese Zaghini; Cristian Torri
Journal:  Front Bioeng Biotechnol       Date:  2021-02-10

6.  Subcritical Water as a Pre-Treatment of Mixed Microbial Biomass for the Extraction of Polyhydroxyalkanoates.

Authors:  Liane Meneses; Asiyah Esmail; Mariana Matos; Chantal Sevrin; Christian Grandfils; Susana Barreiros; Maria A M Reis; Filomena Freitas; Alexandre Paiva
Journal:  Bioengineering (Basel)       Date:  2022-07-08

7.  Improvement of the Polyhydroxyalkanoates Recovery from Mixed Microbial Cultures Using Sodium Hypochlorite Pre-Treatment Coupled with Solvent Extraction.

Authors:  Gabriela Montiel-Jarillo; Diego A Morales-Urrea; Edgardo M Contreras; Alex López-Córdoba; Edwin Yesid Gómez-Pachón; Julián Carrera; María Eugenia Suárez-Ojeda
Journal:  Polymers (Basel)       Date:  2022-09-21       Impact factor: 4.967

8.  Enhanced production of poly(3-hydroxybutyrate) in recombinant Escherichia coli and EDTA-microwave-assisted cell lysis for polymer recovery.

Authors:  Aneesh Balakrishna Pillai; Arjun Jaya Kumar; Harikrishnan Kumarapillai
Journal:  AMB Express       Date:  2018-09-04       Impact factor: 3.298

  8 in total

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