Mária Dux1, Christine Will2, Birgit Vogler2, Milos R Filipovic3, Karl Messlinger2. 1. Department of Physiology, University of Szeged, Szeged, Hungary. 2. Institute of Physiology and Pathophysiology, University of Erlangen-Nürnberg, Erlangen, Germany. 3. Department of Chemistry and Pharmacy, University of Erlangen-Nürnberg, Erlangen, Germany.
Abstract
BACKGROUND AND PURPOSE: Meningeal blood flow is controlled by CGRP released from trigeminal afferents and NO mainly produced in arterial endothelium. The vasodilator effect of NO may be due to the NO-derived compound, nitroxyl (HNO), generated through reaction with endogenous H2 S. We investigated the involvement of HNO in CGRP release and meningeal blood flow. EXPERIMENTAL APPROACH: Blood flow in exposed dura mater of rats was recorded by laser Doppler flowmetry. CGRP release from the dura mater in the hemisected rat head was quantified using an elisa. NO and H2 S were localized histochemically with specific sensors. KEY RESULTS: Topical administration of the NO donor diethylamine-NONOate increased meningeal blood flow by 30%. Pretreatment with oxamic acid, an inhibitor of H2 S synthesis, reduced this effect. Administration of Na2 S increased blood flow by 20%, an effect abolished by the CGRP receptor antagonist CGRP8-37 or the TRPA1 channel antagonist HC030031 and reduced when endogenous NO synthesis was blocked. Na2 S dose-dependently increased CGRP release two- to threefold. Co-administration of diethylamine-NONOate facilitated CGRP release, while inhibition of endogenous NO or H2 S synthesis lowered basal CGRP release. NO and H2 S were mainly localized in arterial vessels, HNO additionally in nerve fibre bundles. HNO staining was lost after treatment with L-NMMA and oxamic acid. CONCLUSIONS AND IMPLICATIONS: NO and H2 S cooperatively increased meningeal blood flow by forming HNO, which activated TRPA1 cation channels in trigeminal fibres, inducing CGRP release. This HNO-TRPA1-CGRP signalling pathway may be relevant to the pathophysiology of headaches.
BACKGROUND AND PURPOSE: Meningeal blood flow is controlled by CGRP released from trigeminal afferents and NO mainly produced in arterial endothelium. The vasodilator effect of NO may be due to the NO-derived compound, nitroxyl (HNO), generated through reaction with endogenous H2 S. We investigated the involvement of HNO in CGRP release and meningeal blood flow. EXPERIMENTAL APPROACH: Blood flow in exposed dura mater of rats was recorded by laser Doppler flowmetry. CGRP release from the dura mater in the hemisected rat head was quantified using an elisa. NO and H2 S were localized histochemically with specific sensors. KEY RESULTS: Topical administration of the NO donordiethylamine-NONOate increased meningeal blood flow by 30%. Pretreatment with oxamic acid, an inhibitor of H2 S synthesis, reduced this effect. Administration of Na2 S increased blood flow by 20%, an effect abolished by the CGRP receptor antagonist CGRP8-37 or the TRPA1 channel antagonist HC030031 and reduced when endogenous NO synthesis was blocked. Na2 S dose-dependently increased CGRP release two- to threefold. Co-administration of diethylamine-NONOate facilitated CGRP release, while inhibition of endogenous NO or H2 S synthesis lowered basal CGRP release. NO and H2 S were mainly localized in arterial vessels, HNO additionally in nerve fibre bundles. HNO staining was lost after treatment with L-NMMA and oxamic acid. CONCLUSIONS AND IMPLICATIONS: NO and H2 S cooperatively increased meningeal blood flow by forming HNO, which activated TRPA1 cation channels in trigeminal fibres, inducing CGRP release. This HNO-TRPA1-CGRP signalling pathway may be relevant to the pathophysiology of headaches.
Authors: Mirjam Eberhardt; Maria Dux; Barbara Namer; Jan Miljkovic; Nada Cordasic; Christine Will; Tatjana I Kichko; Jeanne de la Roche; Michael Fischer; Sebastián A Suárez; Damian Bikiel; Karola Dorsch; Andreas Leffler; Alexandru Babes; Angelika Lampert; Jochen K Lennerz; Johannes Jacobi; Marcelo A Martí; Fabio Doctorovich; Edward D Högestätt; Peter M Zygmunt; Ivana Ivanovic-Burmazovic; Karl Messlinger; Peter Reeh; Milos R Filipovic Journal: Nat Commun Date: 2014-07-15 Impact factor: 14.919
Authors: Weiwei An; Lucas S Ryan; Audrey G Reeves; Kevin J Bruemmer; Lyn Mouhaffel; Jeni L Gerberich; Alexander Winters; Ralph P Mason; Alexander R Lippert Journal: Angew Chem Int Ed Engl Date: 2018-12-21 Impact factor: 15.336
Authors: Alla B Salmina; Yulia K Komleva; István A Szijártó; Yana V Gorina; Olga L Lopatina; Galina E Gertsog; Milos R Filipovic; Maik Gollasch Journal: Front Physiol Date: 2015-12-11 Impact factor: 4.566