Chung-Po Ko1, Chiao-Wen Lin2, Mu-Kuan Chen3, Shun-Fa Yang4, Hui-Ling Chiou5, Ming-Ju Hsieh6. 1. Department of neurosurgery, Tungs' Taichung Metro Harbor Hospital, Taichung, Taiwan; Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan. 2. Institute of Oral Sciences, Chung Shan Medical University, Taichung 40201, Taiwan; Department of Dentistry, Chung Shan Medical University Hospital, Taichung 40201, Taiwan. 3. Department of Otorhinolaryngology-Head and Neck Surgery, Changhua Christian Hospital, Changhua 500, Taiwan. 4. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, Taichung 40201, Taiwan. 5. School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40201, Taiwan; Department of Clinical Laboratory, Chung Shan Medical University Hospital, Taichung 40201, Taiwan. Electronic address: hlchiou@csmu.edu.tw. 6. Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan; Cancer Research Center, Changhua Christian Hospital, Changhua 500, Taiwan; School of Optometry, Chung Shan Medical University, Taichung 40201, Taiwan. Electronic address: 170780@cch.org.tw.
Abstract
OBJECTIVES: Extensive research supports the administration of herbal medicines or natural foods during cancer therapy. Pterostilbene, a naturally occurring phytoalexin, has various pharmacological activities, including antioxidant activity, cancer prevention activity, and cytotoxicity to many cancers. However, the effect of pterostilbene on the autophagy of tumor cells has not been clarified. MATERIALS AND METHODS: In this study, the unique effects of pterostilbene on the autophagy of human oral cancer cells were investigated. RESULTS: The results of this study showed that pterostilbene effectively inhibited the growth of human oral cancer cells by inducing cell cycle arrest and apoptosis. In addition, the formation of acidic vesicular organelles and LC3-II production also demonstrated that pterostilbene induced autophagy. Administering 3-methylamphetamine (3-MA) and bafilomycin A1 (BafA1) exerted differing effects on the pterostilbene-induced death of human oral cancer cells. Pterostilbene-induced autophagy was triggered by activation of JNK1/2 and inhibition of Akt, ERK1/2, and p38. CONCLUSION: In conclusion, this study demonstrated that pterostilbene caused autophagy and apoptosis in human oral cancer cells, suggesting that pterostilbene could serve as a new and promising agent for treating human oral cancer.
OBJECTIVES: Extensive research supports the administration of herbal medicines or natural foods during cancer therapy. Pterostilbene, a naturally occurring phytoalexin, has various pharmacological activities, including antioxidant activity, cancer prevention activity, and cytotoxicity to many cancers. However, the effect of pterostilbene on the autophagy of tumor cells has not been clarified. MATERIALS AND METHODS: In this study, the unique effects of pterostilbene on the autophagy of human oral cancer cells were investigated. RESULTS: The results of this study showed that pterostilbene effectively inhibited the growth of human oral cancer cells by inducing cell cycle arrest and apoptosis. In addition, the formation of acidic vesicular organelles and LC3-II production also demonstrated that pterostilbene induced autophagy. Administering 3-methylamphetamine (3-MA) and bafilomycin A1 (BafA1) exerted differing effects on the pterostilbene-induced death of human oral cancer cells. Pterostilbene-induced autophagy was triggered by activation of JNK1/2 and inhibition of Akt, ERK1/2, and p38. CONCLUSION: In conclusion, this study demonstrated that pterostilbene caused autophagy and apoptosis in human oral cancer cells, suggesting that pterostilbene could serve as a new and promising agent for treating human oral cancer.
Authors: Yi Rong Ivan Lim; Philip M Preshaw; Lum Peng Lim; Marianne Meng Ann Ong; Hai-Shu Lin; Kai Soo Tan Journal: Sci Rep Date: 2020-06-03 Impact factor: 4.379